Document Detail


Opsin1-2, G(q)α and arrestin levels at Limulus rhabdoms are controlled by diurnal light and a circadian clock.
MedLine Citation:
PMID:  23393287     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dark and light adaptation in photoreceptors involve multiple processes including those that change protein concentrations at photosensitive membranes. Light- and dark-adaptive changes in protein levels at rhabdoms have been described in detail in white-eyed Drosophila maintained under artificial light. Here we tested whether protein levels at rhabdoms change significantly in the highly pigmented lateral eyes of wild-caught Limulus polyphemus maintained in natural diurnal illumination and whether these changes are under circadian control. We found that rhabdomeral levels of opsins (Ops1-2), the G protein activated by rhodopsin (G(q)α) and arrestin change significantly from day to night and that nighttime levels of each protein at rhabdoms are significantly influenced by signals from the animal's central circadian clock. Clock input at night increases Ops1-2 and G(q)α and decreases arrestin levels at rhabdoms. Clock input is also required for a rapid decrease in rhabdomeral Ops1-2 beginning at sunrise. We found further that dark adaptation during the day and the night are not equivalent. During daytime dark adaptation, when clock input is silent, the increase of Ops1-2 at rhabdoms is small and G(q)α levels do not increase. However, increases in Ops1-2 and G(q)α at rhabdoms are enhanced during daytime dark adaptation by treatments that elevate cAMP in photoreceptors, suggesting that the clock influences dark-adaptive increases in Ops1-2 and G(q)α at Limulus rhabdoms by activating cAMP-dependent processes. The circadian regulation of Ops1-2 and G(q)α levels at rhabdoms probably has a dual role: to increase retinal sensitivity at night and to protect photoreceptors from light damage during the day.
Authors:
Barbara-Anne Battelle; Karen E Kempler; Alexander K Parker; Cristina D Gaddie
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-02-07
Journal Detail:
Title:  The Journal of experimental biology     Volume:  216     ISSN:  1477-9145     ISO Abbreviation:  J. Exp. Biol.     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-06-20     Completed Date:  2014-01-15     Revised Date:  2014-08-04    
Medline Journal Info:
Nlm Unique ID:  0243705     Medline TA:  J Exp Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1837-49     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Animals
Arrestin / metabolism*
Circadian Clocks
Circadian Rhythm / radiation effects*
Colforsin / pharmacology
Compound Eye, Arthropod / cytology,  drug effects,  metabolism,  radiation effects
Cyclic AMP / metabolism
Dark Adaptation / drug effects,  radiation effects
Darkness
GTP-Binding Protein alpha Subunits, Gq-G11 / metabolism*
Horseshoe Crabs / metabolism*,  radiation effects*
Light*
Octopamine / pharmacology
Opsins / metabolism*
Retina / cytology,  drug effects,  metabolism,  radiation effects
Rhodopsin / metabolism
Grant Support
ID/Acronym/Agency:
P30 EY021721/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/Arrestin; 0/Opsins; 14O50WS8JD/Octopamine; 1F7A44V6OU/Colforsin; 9009-81-8/Rhodopsin; E0399OZS9N/Cyclic AMP; EC 3.6.5.1/GTP-Binding Protein alpha Subunits, Gq-G11
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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