Document Detail


Oppositely imprinted genes H19 and insulin-like growth factor 2 are coexpressed in human androgenetic trophoblast.
MedLine Citation:
PMID:  7692725     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human uniparental gestations such as gynogenetic ovarian teratomas and androgenetic complete hydatidiform moles provide a model to evaluate the integrity of parent-specific gene expression--i.e., imprinting--in the absence of a complementary parental genetic contribution. We studied expression, in these tissues, of the oppositely imprinted genes H19, which is an embryonic nontranslated RNA, and insulin-like growth factor type 2 (IGF2). Normal gestations only express H19 from the maternal allele and express IGF2 from the paternal allele, whereas neither is expressed from the maternal genome of gynogenetic gestations, and both are expressed from the paternal genome of androgenetic gestations. Coexpression of H19 and IGF2 in the androgenetic tissues was in a single population of cells, mononuclear trophoblast--the same cell type expressing these genes in biparental placentas. These results demonstrate that a biparental genome may be required for expression of the reciprocal IGF2/H19 imprint. Alternatively, biparental expression may be a normal feature of some imprinted genes in specific cell types. Additional experiments with other imprinted genes will clarify whether this reflects global failure of the imprinting process or a change specific to the IGF2/H19 locus.
Authors:
G L Mutter; C L Stewart; M L Chaponot; R J Pomponio
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of human genetics     Volume:  53     ISSN:  0002-9297     ISO Abbreviation:  Am. J. Hum. Genet.     Publication Date:  1993 Nov 
Date Detail:
Created Date:  1993-11-19     Completed Date:  1993-11-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370475     Medline TA:  Am J Hum Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1096-102     Citation Subset:  IM    
Affiliation:
Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Blotting, Northern
DNA Probes
Female
Gene Expression
Humans
Hydatidiform Mole / genetics
In Situ Hybridization
Insulin-Like Growth Factor II / biosynthesis,  genetics*
Molecular Sequence Data
Organ Specificity / genetics
Ovarian Neoplasms / genetics
Pregnancy
RNA / biosynthesis,  genetics*
Teratoma / genetics
Trophoblasts / metabolism*
Chemical
Reg. No./Substance:
0/DNA Probes; 63231-63-0/RNA; 67763-97-7/Insulin-Like Growth Factor II
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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