| Opposite effects of nitric oxide on rod and cone photoreceptors of rat retina in situ. | |
| | |
MedLine Citation:
|
PMID: 20171265 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In the light-adapted vertebrate retina, nitric oxide (NO) modulates synaptic transmission between photoreceptors and second-order neurons. Although NO is believed to be a mediator of adaptation, its effect on photoreceptors in situ is not known yet. Therefore, we studied rod and cone activities in rat eyes in situ, using the electroretinogram (ERG). Rod and cone ERGs were functionally isolated by intravitreal 20mM glutamate, which suppressed the activity of all retinal cells except rods and cones for about 90min. The addition of NO-donor, SNAP, to the glutamate solution decreased the amplitude of the rod single-flash ERG by approximately 40%, compared to the amplitude of the rod ERG isolated by glutamate alone, but it increased the amplitude of the isolated, intense paired-flash cone ERG by approximately 40%. An excess of the NO-scavenger, CPTIO, had no significant effect on either rod or cone ERGs. A broad-spectrum NO-synthase inhibitor, L-NAME, increased the amplitude of the rod ERG by approximately 50%, but had no significant effect on the cone ERG. We suggest that NO directly modulates the light-evoked activity of rod and cone photoreceptors in situ, but in opposite ways. |
| | |
Authors:
|
Masaki Sato; Teruya Ohtsuka |
Related Documents
:
|
16547945 - Why photoreceptors die (and why they don't). 16121275 - Molecular properties of rod and cone visual pigments from purified chicken cone pigment... 11425675 - Delays in rod-mediated dark adaptation in early age-related maculopathy. 6308225 - A sign-reversing pathway from rods to double and single cones in the retina of the tige... 21117775 - Temporary threshold shift in a bottlenose dolphin (tursiops truncatus) exposed to inter... 15639505 - Stereothresholds for moving line stimuli for a range of velocities. |
Publication Detail:
|
Type: Journal Article Date: 2010-02-18 |
Journal Detail:
|
Title: Neuroscience letters Volume: 473 ISSN: 1872-7972 ISO Abbreviation: Neurosci. Lett. Publication Date: 2010 Mar |
Date Detail:
|
Created Date: 2010-03-19 Completed Date: 2010-06-17 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: Ireland |
Other Details:
|
Languages: eng Pagination: 62-6 Citation Subset: IM |
Copyright Information:
|
Copyright 2010 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
|
Biology Research Division, Graduate School of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Benzoates / pharmacology Electroretinography Imidazoles / pharmacology Male NG-Nitroarginine Methyl Ester / pharmacology Nitric Oxide / physiology* Nitric Oxide Donors / pharmacology Nitric Oxide Synthase / antagonists & inhibitors Rats Rats, Sprague-Dawley Retinal Cone Photoreceptor Cells / drug effects, physiology* Retinal Rod Photoreceptor Cells / drug effects, physiology* S-Nitroso-N-Acetylpenicillamine / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Benzoates; 0/Imidazoles; 0/Nitric Oxide Donors; 10102-43-9/Nitric Oxide; 145757-47-7/1,3-dihydroxy-4,4,5,5-tetramethyl-2-(4-carboxyphenyl)tetrahydroimidazole; 50903-99-6/NG-Nitroarginine Methyl Ester; 79032-48-7/S-Nitroso-N-Acetylpenicillamine; EC 1.14.13.39/Nitric Oxide Synthase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Expiration: the moment we experience retronasal olfaction in flavor.
Next Document: CRTAC1 homolog proteins are conserved from cyanobacteria to man and secreted by the teleost fish pit...