Document Detail

Opioid peptides inhibit the action of oestradiol on human myometrial cells in culture.
MedLine Citation:
PMID:  10341005     Owner:  NLM     Status:  MEDLINE    
The effect of opioid peptides on cultured, oestradiol-stimulated human myometrial cells was examined. Oestradiol increased cell densities in mixed-cell (smooth muscle cells + stromal fibroblasts) cultures by 40%. This oestradiol-induced stimulation of cell proliferation was decreased to control values by D-met2-pro5-enkephalinamide. The half-effective inhibitory concentration of enkephalinamide was 0.3 nmol/l. The opioid-induced inhibition of cell proliferation was blocked completely by the specific opiate receptor antagonist naloxone, while naloxone did not have any effect on its own. This opioid effect was mediated dominantly by the mu opiate receptor. The optimal concentration for oestradiol to stimulate uterine cell proliferation was 2.2 nM. The basal rate of cell proliferation was not affected by enkephalinamide. In saturation experiments, the parameters of specific [3H]-naloxone binding were: dissociation constant = 1.02 nM, maximal binding capacity = 2910 binding sites/cell, Hill coefficient = 1.029. In human myometrial pure smooth muscle cell cultures, oestradiol decreased the proliferation of cells. Progesterone potentiated these oestradiol effects, but had no effect on its own. Enkephalinamide was also able to block the effects of oestradiol, but naloxone did not antagonize it. In summary, here we present a novel inhibitory role of endogenous opioid peptides in the regulation of cell growth and proliferation in the human uterus.
J L Környei; Z Vértes; A Oszter; K A Kovács; C V Rao; M Vértes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular human reproduction     Volume:  5     ISSN:  1360-9947     ISO Abbreviation:  Mol. Hum. Reprod.     Publication Date:  1999 Jun 
Date Detail:
Created Date:  1999-07-20     Completed Date:  1999-07-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9513710     Medline TA:  Mol Hum Reprod     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  565-72     Citation Subset:  IM    
Institute of Physiology, and Department of Obstetrics & Gynecology, University Medical School of Pécs, Pécs, Szigeti str. 12, H-7643 Hungary.
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MeSH Terms
Cell Division / drug effects,  physiology
Cells, Cultured
Dynorphins / pharmacology
Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
Enkephalin, D-Penicillamine (2,5)-
Enkephalin, Methionine / analogs & derivatives,  pharmacology
Enkephalins / metabolism*,  pharmacology
Estradiol / metabolism*
Inhibitory Concentration 50
Muscle, Smooth / cytology,  drug effects,  metabolism
Myometrium / cytology,  drug effects,  metabolism*
Naloxone / pharmacology
Narcotic Antagonists / pharmacology
Progesterone / pharmacology
Grant Support
Reg. No./Substance:
0/Enkephalins; 0/Narcotic Antagonists; 100929-53-1/Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; 465-65-6/Naloxone; 50-28-2/Estradiol; 57-83-0/Progesterone; 58569-55-4/Enkephalin, Methionine; 63307-63-1/enkephalin, Met(2)-ProNH2(5)-; 74913-18-1/Dynorphins; 88373-73-3/Enkephalin, D-Penicillamine (2,5)-

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