Document Detail

Opioid peptides in the nervous system of Aplysia: a combined biochemical, immunocytochemical, and electrophysiological study.
MedLine Citation:
PMID:  8590454     Owner:  NLM     Status:  MEDLINE    
1. We have used biochemical, immunocytochemical, and electrophysiological techniques to evaluate the role of opioid peptides in the central nervous system of the marine mollusc, Aplysia californica. 2. Binding studies using 3H-D-Ala2, met-enkephalinamide (3H-DAMA) showed a single class of high-affinity binding sites with a Kd of 1.3 nM and a binding density of 45 pmol/g. 3. HPLC extracts of ganglia revealed multiple peaks with immunoreactivity for either leu (LEU-IR)- or met-enkephalin (MET-IR), but the amounts were not uniformly distributed in all ganglia. 4. LEU-IR and MET-IR neurons were demonstrated immunocytochemically in all ganglia, but MET-IR neurons were more frequent and were concentrated in pedal and pleural ganglia. While absorption control studies abolished MET-IR, LEU-IR was only partially abolished in the neuropil. 5. In electrophysiological studies, both depolarizing and hyperpolarizing responses were found to D-Ala2-leu-enkephalin (DALEU) and D-Ala2-met enkephalin (DAMET) on some and different neurons. 6. HPLC fractions from regions with retention times corresponding to authentic leu- or met-enkephalin showed physiologic responses similar to those of DALEU and DAMET, respectively. 7. These studies suggest that a variety of endogeneous opioid peptides play physiologically important roles in the nervous system of Aplysia, including but not necessarily limited to leu- and met-enkephalin.
D O Carpenter; G Kemenes; K Elekes; M Leung; G Stefano; K S Rózsa; J Salánki
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cellular and molecular neurobiology     Volume:  15     ISSN:  0272-4340     ISO Abbreviation:  Cell. Mol. Neurobiol.     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1996-03-25     Completed Date:  1996-03-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8200709     Medline TA:  Cell Mol Neurobiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  239-56     Citation Subset:  IM    
Wadsworth Center for Laboratories and Research, New York State Department of Health and School of Public Health, Albany 12201, USA.
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MeSH Terms
Analgesics, Opioid / pharmacology
Dose-Response Relationship, Drug
Electrophysiology / methods
Enkephalin, Leucine / analysis*
Enkephalin, Leucine-2-Alanine / analogs & derivatives,  pharmacology
Enkephalin, Methionine / analogs & derivatives,  analysis*,  metabolism,  pharmacology
Ganglia, Invertebrate / cytology*,  physiology*
Immunohistochemistry / methods
Membrane Potentials / drug effects
Naloxone / pharmacology
Narcotic Antagonists / pharmacology
Neurons / cytology,  drug effects,  physiology*
Organ Specificity
Patch-Clamp Techniques
Receptors, Opioid / metabolism
Grant Support
Reg. No./Substance:
0/Analgesics, Opioid; 0/Narcotic Antagonists; 0/Receptors, Opioid; 465-65-6/Naloxone; 58569-55-4/Enkephalin, Methionine; 58822-25-6/Enkephalin, Leucine; 60117-17-1/Met-enkephalinamide; 61370-87-4/enkephalin-Met, Ala(2)-; 63631-40-3/Enkephalin, Leucine-2-Alanine; 65189-64-2/enkephalinamide-Leu, Ala(2)-

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