Document Detail


Opioid peptides in the nervous system of Aplysia: a combined biochemical, immunocytochemical, and electrophysiological study.
MedLine Citation:
PMID:  8590454     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. We have used biochemical, immunocytochemical, and electrophysiological techniques to evaluate the role of opioid peptides in the central nervous system of the marine mollusc, Aplysia californica. 2. Binding studies using 3H-D-Ala2, met-enkephalinamide (3H-DAMA) showed a single class of high-affinity binding sites with a Kd of 1.3 nM and a binding density of 45 pmol/g. 3. HPLC extracts of ganglia revealed multiple peaks with immunoreactivity for either leu (LEU-IR)- or met-enkephalin (MET-IR), but the amounts were not uniformly distributed in all ganglia. 4. LEU-IR and MET-IR neurons were demonstrated immunocytochemically in all ganglia, but MET-IR neurons were more frequent and were concentrated in pedal and pleural ganglia. While absorption control studies abolished MET-IR, LEU-IR was only partially abolished in the neuropil. 5. In electrophysiological studies, both depolarizing and hyperpolarizing responses were found to D-Ala2-leu-enkephalin (DALEU) and D-Ala2-met enkephalin (DAMET) on some and different neurons. 6. HPLC fractions from regions with retention times corresponding to authentic leu- or met-enkephalin showed physiologic responses similar to those of DALEU and DAMET, respectively. 7. These studies suggest that a variety of endogeneous opioid peptides play physiologically important roles in the nervous system of Aplysia, including but not necessarily limited to leu- and met-enkephalin.
Authors:
D O Carpenter; G Kemenes; K Elekes; M Leung; G Stefano; K S Rózsa; J Salánki
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cellular and molecular neurobiology     Volume:  15     ISSN:  0272-4340     ISO Abbreviation:  Cell. Mol. Neurobiol.     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1996-03-25     Completed Date:  1996-03-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8200709     Medline TA:  Cell Mol Neurobiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  239-56     Citation Subset:  IM    
Affiliation:
Wadsworth Center for Laboratories and Research, New York State Department of Health and School of Public Health, Albany 12201, USA.
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MeSH Terms
Descriptor/Qualifier:
Analgesics, Opioid / pharmacology
Animals
Aplysia
Dose-Response Relationship, Drug
Electrophysiology / methods
Enkephalin, Leucine / analysis*
Enkephalin, Leucine-2-Alanine / analogs & derivatives,  pharmacology
Enkephalin, Methionine / analogs & derivatives,  analysis*,  metabolism,  pharmacology
Ganglia, Invertebrate / cytology*,  physiology*
Immunohistochemistry / methods
Membrane Potentials / drug effects
Naloxone / pharmacology
Narcotic Antagonists / pharmacology
Neurons / cytology,  drug effects,  physiology*
Organ Specificity
Patch-Clamp Techniques
Receptors, Opioid / metabolism
Grant Support
ID/Acronym/Agency:
NIDA 09019/DA/NIDA NIH HHS
Chemical
Reg. No./Substance:
0/Analgesics, Opioid; 0/Narcotic Antagonists; 0/Receptors, Opioid; 465-65-6/Naloxone; 58569-55-4/Enkephalin, Methionine; 58822-25-6/Enkephalin, Leucine; 60117-17-1/Met-enkephalinamide; 61370-87-4/enkephalin-Met, Ala(2)-; 63631-40-3/Enkephalin, Leucine-2-Alanine; 65189-64-2/enkephalinamide-Leu, Ala(2)-

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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