| Opioid peptides in the nervous system of Aplysia: a combined biochemical, immunocytochemical, and electrophysiological study. | |
| | |
MedLine Citation:
|
PMID: 8590454 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
1. We have used biochemical, immunocytochemical, and electrophysiological techniques to evaluate the role of opioid peptides in the central nervous system of the marine mollusc, Aplysia californica. 2. Binding studies using 3H-D-Ala2, met-enkephalinamide (3H-DAMA) showed a single class of high-affinity binding sites with a Kd of 1.3 nM and a binding density of 45 pmol/g. 3. HPLC extracts of ganglia revealed multiple peaks with immunoreactivity for either leu (LEU-IR)- or met-enkephalin (MET-IR), but the amounts were not uniformly distributed in all ganglia. 4. LEU-IR and MET-IR neurons were demonstrated immunocytochemically in all ganglia, but MET-IR neurons were more frequent and were concentrated in pedal and pleural ganglia. While absorption control studies abolished MET-IR, LEU-IR was only partially abolished in the neuropil. 5. In electrophysiological studies, both depolarizing and hyperpolarizing responses were found to D-Ala2-leu-enkephalin (DALEU) and D-Ala2-met enkephalin (DAMET) on some and different neurons. 6. HPLC fractions from regions with retention times corresponding to authentic leu- or met-enkephalin showed physiologic responses similar to those of DALEU and DAMET, respectively. 7. These studies suggest that a variety of endogeneous opioid peptides play physiologically important roles in the nervous system of Aplysia, including but not necessarily limited to leu- and met-enkephalin. |
| | |
Authors:
|
D O Carpenter; G Kemenes; K Elekes; M Leung; G Stefano; K S Rózsa; J Salánki |
Related Documents
:
|
6086884 - A novel phencyclidine analog interacts selectively with mu opioid receptors. 6290634 - Evidence for sigma opioid receptor: binding of [3h]skf-10047 to etorphine-inaccessible ... 7475964 - Receptor reserve and affinity of mu opioid agonists in mouse antinociception: correlati... 2999404 - Factors affecting binding of trans-n-[2-(methylamino)cyclohexyl]benzamides at the prima... 1859824 - The interaction of [13c]-enriched colchicine with tubulin as determined by nmr spectros... 2158454 - Specific [125i]brain natriuretic peptide-26 binding sites in rat and pig kidneys. |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Cellular and molecular neurobiology Volume: 15 ISSN: 0272-4340 ISO Abbreviation: Cell. Mol. Neurobiol. Publication Date: 1995 Apr |
Date Detail:
|
Created Date: 1996-03-25 Completed Date: 1996-03-25 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 8200709 Medline TA: Cell Mol Neurobiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 239-56 Citation Subset: IM |
Affiliation:
|
Wadsworth Center for Laboratories and Research, New York State Department of Health and School of Public Health, Albany 12201, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Analgesics, Opioid
/
pharmacology Animals Aplysia Dose-Response Relationship, Drug Electrophysiology / methods Enkephalin, Leucine / analysis* Enkephalin, Leucine-2-Alanine / analogs & derivatives, pharmacology Enkephalin, Methionine / analogs & derivatives, analysis*, metabolism, pharmacology Ganglia, Invertebrate / cytology*, physiology* Immunohistochemistry / methods Membrane Potentials / drug effects Naloxone / pharmacology Narcotic Antagonists / pharmacology Neurons / cytology, drug effects, physiology* Organ Specificity Patch-Clamp Techniques Receptors, Opioid / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
NIDA 09019/DA/NIDA NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Analgesics, Opioid; 0/Narcotic Antagonists; 0/Receptors, Opioid; 465-65-6/Naloxone; 58569-55-4/Enkephalin, Methionine; 58822-25-6/Enkephalin, Leucine; 60117-17-1/Met-enkephalinamide; 61370-87-4/enkephalin-Met, Ala(2)-; 63631-40-3/Enkephalin, Leucine-2-Alanine; 65189-64-2/enkephalinamide-Leu, Ala(2)- |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Expression of mRNAs of multiple growth factors and receptors by astrocytes and glioma cells: detecti...
Next Document: Expression of the small heat shock protein (hsp) 27 in human astrocytomas correlates with histologic...