| Opioid-like activity of naltrexone on natural killer cell cytolytic activity and cytokine production in splenocytes: effects of alcohol. | |
| | |
MedLine Citation:
|
PMID: 19929573 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Chronic alcohol consumption has been shown to decrease the activity of natural killer (NK) cell cytolytic function and the production of various cytokines from the spleen. We have recently shown that naltrexone, an opiate receptor antagonist, when administered for a period of 2 weeks suppresses micro-opiate receptor binding but increases partial differential-opiate receptor activity in rat splenocytes. However, the effects of long-term naltrexone treatment on alcohol-induced alteration of NK cell cytolytic activity and cytokines production in splenocytes have not been determined. Male rats were pair-fed an isocaloric liquid diet or fed an ethanol-containing liquid diet for a period of 3 weeks. These rats were additionally treated after a week with a subcutaneous implant of either a naltrexone pellet or placebo pellet for 2 weeks. Splenocytes were isolated and used for determination of various cytokines interleukin (IL)-2, IL-4, and IL-6, and interferon-gamma (IFN-gamma) using enzyme-linked immunosorbent assay (ELISA), and the basal and IL-2-, IL-12-, or IL-18-induced NK cytolytic activity was measured using a standard 4-h (51)Cr release assay against YAC-1 lymphoma target cells. Ethanol consumption resulted in a reduction of the production of IL-2, IL-4, and IL-6 as well as the basal and cytokine-activated NK cell cytolytic activity and IFN-gamma production in splenocytes. Naltrexone administration increased the production of IL-2, IL-4, and IL-6 and the basal and cytokine-activated NK cell cytolytic activity and IFN-gamma production in the splenocytes of pair-fed and alcohol-fed rats. These results indicated that naltrexone treatment increases NK cell cytolytic activity and cytokine production in the spleen in vivo. Furthermore, these results identify the potential of the use of naltrexone in the treatment of immune deficiency in alcoholic and non-alcoholic patients. |
| | |
Authors:
|
Nadka I Boyadjieva; Dipak K Sarkar |
Related Documents
:
|
12053913 - Kinetics of interleukin-2 production in chickens infected with eimeria tenella. 1349533 - Interleukin (il)-4 production by human t cells: differential regulation of il-4 vs. il-... 11493353 - Cytokine gene expression in chronic periodontitis. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research Volume: 30 ISSN: 1557-7465 ISO Abbreviation: J. Interferon Cytokine Res. Publication Date: 2010 Jan |
Date Detail:
|
Created Date: 2010-01-13 Completed Date: 2010-06-10 Revised Date: 2011-07-28 |
Medline Journal Info:
|
Nlm Unique ID: 9507088 Medline TA: J Interferon Cytokine Res Country: United States |
Other Details:
|
Languages: eng Pagination: 15-22 Citation Subset: IM |
Affiliation:
|
Department of Animal Sciences, Rutgers, The State University of New Jersey, Center of Alcohol Studies, New Brunswick, New Jersey 08901, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Administration, Oral Alcoholism / immunology, metabolism Animals Cells, Cultured Cytokines / biosynthesis*, genetics, immunology Cytotoxicity, Immunologic / drug effects Drug Implants Ethanol / toxicity Immunomodulation Infusions, Subcutaneous Killer Cells, Natural / drug effects*, immunology, metabolism, pathology Male Naltrexone / administration & dosage* Rats Recovery of Function Spleen / drug effects*, immunology, pathology |
| Grant Support | |
ID/Acronym/Agency:
|
AA016296/AA/NIAAA NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Cytokines; 0/Drug Implants; 16590-41-3/Naltrexone; 64-17-5/Ethanol |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Association of Interleukin-8 Gene Polymorphism With Cachexia From Patients With Gastric Cancer.
Next Document: Cellular Cholesterol Involvement in Src, PKC, and p38/JNK Transduction Pathways by Porins.