Document Detail

Ophidian kidney preparation for the measurement of glomerular dynamics in real-time.
MedLine Citation:
PMID:  2315009     Owner:  NLM     Status:  MEDLINE    
A new ophidian kidney preparation is described which allows the measurement of glomerular arteriolar diameters, glomerular blood flow, and glomerular capillary pressure in real time. Intravital epifluorescence video microscopy is utilized to observe and record blood flow in the renal microcirculation in the garter snake, Thamnophis sirtalis. Carotid, renal artery and glomerular capillary pressures are recorded digitally on the video recording simultaneously with the images with an eight-channel video data recorder, maintaining synchrony of all data. On the video replay, glomerular arteriolar diameters are measured with a video micrometer and RBC velocity determined with the video dual-slit method. Blood flow is continuously calculated from the diameter and RBC velocity measurements. The continuous and simultaneous measurements of the pressure gradient across the afferent limb of the glomerular circulation, the renal artery to glomerular capillary pressure drop, and the rate of glomerular blood flow allow the continuous calculation of afferent glomerular arteriolar resistance in real time. This is the first demonstration of these capabilities in a vertebrate kidney.
S D Yokota
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Pflügers Archiv : European journal of physiology     Volume:  415     ISSN:  0031-6768     ISO Abbreviation:  Pflugers Arch.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-04-13     Completed Date:  1990-04-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0154720     Medline TA:  Pflugers Arch     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  501-3     Citation Subset:  IM    
Department of Physiology, School of Medicine, West Virginia University, Morgantown 26506.
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MeSH Terms
Glomerular Filtration Rate / physiology
Kidney / blood supply,  physiology*
Renal Circulation / physiology*
Snakes / physiology*
Grant Support

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