Document Detail

Operator-assisted semi-automatic karyotyping of banded metaphases.
MedLine Citation:
PMID:  908202     Owner:  NLM     Status:  MEDLINE    
Computerized chromosome analyses have resulted in up to 25% erroneous classifications, whereas a cytogenetically trained laboratory technician has an error rate of less than 0.1% in the preparation of a karyotype. In the project reported here, computerized classification is eliminated, and instead, the other steps of the analysis leading to the final karyotype are automated. In the instrument is a projection microscope, modified to project the image on paper before the operator. The slide is scanned on a motor-driven scanning table, in search of a suitable metaphase. The operator stops the scanning motor when a suitable metaphase comes into view. The individual chromosomes of the metaphase are then framed, using a view finder, and are identified, after which the chromosome number is entered on a kayboard. The instrument automatically ensures that, for each data entry, the framed chromosome is photographed in such a manner that the chromosome is placed in the proper location with the correct orientation in the karyotype. When all chromosomes have been identified, and the data have been keyed in, a photograph of the finished karyotype is presented. Marking the metaphase chromosomes on the paper, chromosomes are counted within 20 s. With conventional methods, a total chromosome analysis takes 3-6 h, but the above-described device requires 20-30 min.
H C Wulf
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytogenetics and cell genetics     Volume:  19     ISSN:  0301-0171     ISO Abbreviation:  Cytogenet. Cell Genet.     Publication Date:  1977  
Date Detail:
Created Date:  1977-11-30     Completed Date:  1977-11-30     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0367735     Medline TA:  Cytogenet Cell Genet     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  146-58     Citation Subset:  IM    
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MeSH Terms
Karyotyping / instrumentation,  methods*
Time Factors

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