Document Detail

Open pulled straw vitrification of goat embryos at various stages of development.
MedLine Citation:
PMID:  20102780     Owner:  NLM     Status:  MEDLINE    
This investigation addresses the question whether it is possible to apply the open pulled straw (OPS) vitrification method, found to be effective for cryopreserving caprine (Capra aegagrus hircus) blastocysts, to other embryonal stages. Morulae, blastocysts and hatched blastocysts were cryopreserved by way of OPS vitrification and blastocysts and hatched blastocysts by conventional freezing. Morulae were not included with conventional freezing because in our experience the survival rate is very low. To assess the viability of the cryopreserved embryos, they were transferred to synchronized does; in most cases, two embryos per doe. After OPS vitrification, of nine does receiving morulae, not a single one became pregnant; of 11 does receiving blastocysts, nine (82%) became pregnant (all of which kidded and gave birth to, on average, 1.8 kids); and of nine does receiving hatched blastocysts, three (33%) became pregnant (two of which [22%] kidded, giving birth to a single kid each). After conventional freezing, of 10 does receiving blastocysts, five became pregnant (four of which [40%] carried to term and gave birth to a pair of twins each); and of nine does receiving hatched blastocysts, three (33%) became pregnant (and gave birth to a single kid each). Embryo survival (kids born/embryos transferred) after vitrification for morulae, blastocysts, and hatched blastocysts was 0, 70% (16 of 23), and 13% (2 of 16), respectively, and after conventional freezing for blastocysts and hatched blastocysts was 42% (8 of 19) and 19% (3 of 16), respectively. The difference in pregnancy and kidding rate between vitrified and conventionally frozen blastocysts was significant, and so was the difference in pregnancy rate between hatched and nonhatched blastocysts, regardless whether OPS-vitrified or conventionally frozen. The results of the current study indicate that OPS vitrification is a very effective means of cryopreserving caprine blastocysts. Unfortunately, the superiority of OPS vitrification over conventional freezing does not apply to caprine morulae and hatched blastocysts.
A N Al Yacoub; M Gauly; W Holtz
Publication Detail:
Type:  Journal Article; Randomized Controlled Trial     Date:  2010-01-25
Journal Detail:
Title:  Theriogenology     Volume:  73     ISSN:  1879-3231     ISO Abbreviation:  Theriogenology     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-05     Completed Date:  2010-08-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1018-23     Citation Subset:  IM    
Copyright Information:
(c) 2010 Elsevier Inc. All rights reserved.
Department of Animal Science, Georg-August-University, Goettingen, Germany.
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MeSH Terms
Cleavage Stage, Ovum / physiology
Cryopreservation / instrumentation,  methods*
Embryo Transfer
Embryo, Mammalian*
Embryonic Development / physiology*
Goats / embryology*,  physiology
Ovulation Induction / veterinary
Pregnancy Rate
Pregnancy, Animal*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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