Document Detail

Open-pulled straw (OPS) vitrification of in vitro fertilised mouse embryos at various stages.
MedLine Citation:
PMID:  18669252     Owner:  NLM     Status:  MEDLINE    
The present study was designed to investigate the cryotolerance of in vitro fertilised (IVF) mouse embryos at various preimplantation developmental stages. IVF mouse embryos were vitrified by the open-pulled straw (OPS) method. After warming, embryos were morphologically evaluated and assessed by their development to blastocysts, hatched blastocysts or term. The results showed that a high proportion (93.3-100.0%) of vitrified embryos at all developmental stages were morphologically normal after recovery. The developmental rate of vitrified 1-cell embryos to blastocyst (40.0%) or hatched blastocyst (32.7%) or term (9.3%) was significantly lower than that from other stages (P < 0.05). Vitrified embryos from 2-cell to early blastocyst stage showed similar blastocyst (71.8-89.5%) and hatched blastocyst rates (61.1-69.6%) and could develop to term without a significant loss of survival compared with those of fresh embryos (P > 0.05). Vitrified 2-cell embryos showed the highest survival rate in vivo (50.6%, 88/174), compared with that from other stages (9.3-30.5%, P < 0.05). The data demonstrate that the OPS method is suitable for the cryopreservation of IVF mouse embryos from 2-cell stage to early blastocyst stage without a significant loss of survival. Embryos at the 2-cell stage had the best tolerance for cryopreservation in the present study.
Chang-Liang Yan; Qi-En Yang; Guang-Bin Zhou; Yun-Peng Hou; Xue-Ming Zhao; Zhi-Qiang Fan; Man-Qing Liu; Lin Liu; Shi-En Zhu
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta veterinaria Hungarica     Volume:  56     ISSN:  0236-6290     ISO Abbreviation:  Acta Vet. Hung.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-08-01     Completed Date:  2008-10-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406376     Medline TA:  Acta Vet Hung     Country:  Hungary    
Other Details:
Languages:  eng     Pagination:  245-53     Citation Subset:  IM    
College of Animal Science and Technology, China Agricultural University, Beijing, PR China.
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MeSH Terms
Animals, Newborn
Cryopreservation / methods,  veterinary*
Embryo Transfer / veterinary
Fertilization in Vitro / methods,  veterinary*
Mice / embryology*

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