Document Detail

Oocyte regulation of anti-Müllerian hormone expression in granulosa cells during ovarian follicle development in mice.
MedLine Citation:
PMID:  14729489     Owner:  NLM     Status:  MEDLINE    
In the ovarian follicle, anti-Müllerian hormone (Amh) mRNA is expressed in granulosa cells from primary to preovulatory stages but becomes restricted to cumulus cells following antrum formation. Anti-Müllerian hormone regulates follicle development by attenuating the effects of follicle stimulating hormone on follicle growth and inhibiting primordial follicle recruitment. To examine the role of the oocyte in regulating granulosa cell Amh expression in the mouse, isolated oocytes and granulosa cells were co-cultured and Amh mRNA levels were analysed by real-time RT-PCR. Expression in freshly isolated granulosa cells increased with preantral follicle development but was low in the cumulus and virtually absent in the mural granulosa cells of preovulatory follicles. When preantral granulosa cells were co-cultured with oocytes from early preantral, late preantral or preovulatory follicles, and when oocytes from preovulatory follicles were co-cultured with cumulus granulosa cells, Amh expression was increased at least 2-fold compared with granulosa cells cultured alone. With oocytes from preantral but not preovulatory follicles, this was a short-range effect only observed with granulosa cells in close apposition to oocytes. We conclude that stage-specific oocyte regulation of Amh expression may play a role in intra- and inter-follicular coordination of follicle development.
Nicholas A Salmon; Alan H Handyside; Ieuan M Joyce
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental biology     Volume:  266     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-01-19     Completed Date:  2004-03-30     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  201-8     Citation Subset:  IM    
School of Biology, University of Leeds, Leeds LS2 9JT, UK.
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MeSH Terms
Anti-Mullerian Hormone
Base Sequence
DNA Primers
Glycoproteins / genetics*
Granulosa Cells / metabolism*
Oocytes / physiology*
RNA, Messenger / genetics
Testicular Hormones / genetics*
Reg. No./Substance:
0/DNA Primers; 0/Glycoproteins; 0/RNA, Messenger; 0/Testicular Hormones; 80497-65-0/Anti-Mullerian Hormone

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