Document Detail


Oocyte maturation and in vitro hormone production in small antral follicles (SAFs) isolated from rhesus monkeys.
MedLine Citation:
PMID:  23423613     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: The small antral follicles (SAFs) from the ovarian medulla can be a potential source of oocytes for infertility patients, but little is known about their ability to yield mature oocytes. This study evaluated the response of these SAFs to a stimulatory bolus of human corionic gonadotropin (hCG) in vitro.
METHODS: Oocyte nuclear maturation and hormone production (estradiol [E2], progesterone [P4]), antimullerian hormone [AMH]) by individual intact SAFs (n = 91; >0.5 mm; n = 5 monkeys) was evaluated after 34 h of culture in the absence (control) or presence of hCG.
RESULTS: Of the total cohort (n = 91), 49 % of SAFs contained degenerating oocytes. The percentage of healthy oocytes able to reinitiate meiosis to the metaphase I (MI) and MII was greater (p < 0.05) after hCG compared to controls. E2, P4 and AMH levels were higher (p < 0.05) in SAF cultures containing germinal vesicle (GV) oocytes compared to those with MII oocytes regardless of hCG exposure. SAF with MI oocytes produced more E2, but less (p < 0.05) P4 and AMH compared to SAFs containing GV oocytes (p < 0.05). Follicles ≥1 mm produced more (p < 0.05) E2, whereas follicle diameter did not correlate with P4 or AMH levels. Only P4 increased (p < 0.05) in response to hCG, regardless of follicle size or oocyte maturity. SAFs containing degenerating oocytes produced similar levels of E2, P4 and AMH compared to SAFs containing healthy oocytes.
CONCLUSIONS: These data indicate, for the first time, that oocytes within primate SAFs can reinitiate meiosis in vitro in the absence of hCG, but nuclear maturation is enhanced in SAFs cultured with hCG. Oocyte nuclear maturation within SAFs in is associated with decreased E2, P4 and AMH levels. Furthermore, hormone content within the culture media does not necessarily reflect oocyte quality.
Authors:
Marina C Peluffo; Jon D Hennebold; Richard L Stouffer; Mary B Zelinski
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-02-20
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  30     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-26     Completed Date:  2013-09-23     Revised Date:  2014-04-01    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  353-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Mullerian Hormone / metabolism
Estradiol / metabolism
Female
Fertilization in Vitro
Gonadotropins / metabolism
Humans
In Vitro Oocyte Maturation Techniques*
Macaca mulatta / growth & development*
Meiosis
Oocytes / cytology*,  growth & development
Oogenesis / drug effects
Ovarian Follicle / cytology*,  growth & development
Pregnancy
Progesterone / metabolism
Grant Support
ID/Acronym/Agency:
1 UL1 RR024926/RR/NCRR NIH HHS; PL1-EB008542/EB/NIBIB NIH HHS; R01 HD052909/HD/NICHD NIH HHS; R01-HD058294/HD/NICHD NIH HHS; RR000163/RR/NCRR NIH HHS; U54 HD055744/HD/NICHD NIH HHS; U54 HD18185/HD/NICHD NIH HHS; U54 HD55744/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Gonadotropins; 4G7DS2Q64Y/Progesterone; 4TI98Z838E/Estradiol; 80497-65-0/Anti-Mullerian Hormone
Comments/Corrections

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