Document Detail

Oocyte-like structures arising from cells of follicular fluid are not captured in aspirates.
MedLine Citation:
PMID:  19298748     Owner:  NLM     Status:  MEDLINE    
Recently, cells from ovarian surface epithelium (OSE) of post-menopausal women and women with premature ovarian failure were investigated and oocyte-like cells with diameters up to 95 microm were found to arise after a certain time in culture. In addition, it seems that a mixed population of germ cells and germline stem cells exists in non-follicle ovarian structures. Relating to an earlier publication, where it was shown that pre-antral follicles with immature oocytes could be captured in follicular fluid (FF) aspirates due to the incorporated tissue in the puncture needle, it was reasoned that OSE or otherwise germline stem cells, possibly captured equally through ovarian puncture, might give rise to oocyte-like cells. The aim of this study was therefore to try to derive such oocyte-like cells from FF aspirates of patients undergoing IVF after culture. Additionally, FF-derived cells were aggregated with human embryonic stem cells to see if an embryonic environment had the ability to enable cells from the FF aspirate to acquire an oocyte-like morphology. Investigations could not confirm the development of oocyte-like cells from cells of FF aspirates.
Nicolas H Zech; Artem Shkumatov; Yury Verlinsky
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  18     ISSN:  1472-6491     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-20     Completed Date:  2009-05-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  443-8     Citation Subset:  IM    
Reproductive Genetics Institute, 2825 N Halsted, Chicago, IL 60657, USA.
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MeSH Terms
Cell Line
Fertilization in Vitro
Follicular Fluid / cytology*

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