Document Detail


Oocyte-like structures arising from cells of follicular fluid are not captured in aspirates.
MedLine Citation:
PMID:  19298748     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recently, cells from ovarian surface epithelium (OSE) of post-menopausal women and women with premature ovarian failure were investigated and oocyte-like cells with diameters up to 95 microm were found to arise after a certain time in culture. In addition, it seems that a mixed population of germ cells and germline stem cells exists in non-follicle ovarian structures. Relating to an earlier publication, where it was shown that pre-antral follicles with immature oocytes could be captured in follicular fluid (FF) aspirates due to the incorporated tissue in the puncture needle, it was reasoned that OSE or otherwise germline stem cells, possibly captured equally through ovarian puncture, might give rise to oocyte-like cells. The aim of this study was therefore to try to derive such oocyte-like cells from FF aspirates of patients undergoing IVF after culture. Additionally, FF-derived cells were aggregated with human embryonic stem cells to see if an embryonic environment had the ability to enable cells from the FF aspirate to acquire an oocyte-like morphology. Investigations could not confirm the development of oocyte-like cells from cells of FF aspirates.
Authors:
Nicolas H Zech; Artem Shkumatov; Yury Verlinsky
Related Documents :
6423008 - The calcium-calmodulin system: participation in the regulation of steroidogenesis at di...
2628738 - Expression of basic fibroblast growth factor in the rat ovary: detection of mrna using ...
12885568 - Drosophila filamin is required for follicle cell motility during oogenesis.
8370918 - Endothelin-1 is an autocrine/paracrine regulator of porcine granulosa cells.
1225718 - Potentiation of cytostatic effect of sulfur- or chlorine-containing purines and related...
12930718 - Germ cell transplantation in an azoospermic klinefelter bull.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  18     ISSN:  1472-6491     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-20     Completed Date:  2009-05-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  443-8     Citation Subset:  IM    
Affiliation:
Reproductive Genetics Institute, 2825 N Halsted, Chicago, IL 60657, USA. n.zech@ivf.at
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Line
Female
Fertilization in Vitro
Follicular Fluid / cytology*
Humans
Oocytes*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Metformin and atorvastatin reduce adhesion formation in a rat uterine horn model.
Next Document:  Hyperbaric oxygen therapy and cerebral ischemia: neuroprotective mechanisms.