| Oocyte cryopreservation outcomes including pre-cryopreservation and post-thaw meiotic spindle evaluation following slow cooling and vitrification of human oocytes. | |
| | |
MedLine Citation:
|
PMID: 20188356 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: To report our oocyte cryopreservation (OC) outcomes including meiotic spindle (MS) evaluation of metaphase II (MII) oocytes destined for OC and thaw. DESIGN: Retrospective. SETTING: University-based infertility center. PATIENT(S): Women attempting pregnancy using cryopreserved oocytes. INTERVENTION(S): OC, MS evaluation. MAIN OUTCOME MEASURE(S): Survival, two pronuclear (2PN) fertilization, achieving embryo quality suitable for transfer or refreezing, blastocyst formation. RESULT(S): Thirty-two OC-thaw cycles resulted in 20 pregnancies, 18 either ongoing or delivered. In 26 cycles, MS evaluation was performed: 262/303 (86%) thawed/recovered oocytes survived, 218/262 (83%) achieved 2PN fertilization, 133/218 (61%) became suitable for day-3 and 122/218 (56%) for day-5 transfer. In total, 58 embryos were transferred resulting in a 62% pregnancy and a 41% implantation rate. Of oocytes evaluated before cryopreservation, 247 (82%) were spindle-positive; 96% of these were also spindle-positive after thawing. Blastocyst formation and suitability for day-5 transfer was achieved more often if a post-thaw spindle was visualized. Of all slow-cooled and vitrified oocytes, a higher percentage of those slow-cooled achieved 2PN fertilization and usability. MS evaluation of oocytes cryopreserved by either method was associated with similar outcomes. CONCLUSION(S): OC outcomes are improving. An MS was almost always exhibited both before cryopreservation and after thawing, suggesting that, with appropriate technique, OC presents minimal harm to the MII oocyte. A meiotic spindle evaluation might help to further OC technology. |
| | |
Authors:
|
Nicole Noyes; Jaime Knopman; Patty Labella; Caroline McCaffrey; Melicia Clark-Williams; Jamie Grifo |
Related Documents
:
|
20886366 - Successful live birth from vitrified oocytes after 5 years of cryopreservation. 2442036 - Two years' experience of ultrasound-directed oocyte retrieval. 2508776 - In vitro fertilization and embryo transfer in the rhesus monkey. 19171336 - Gonadotropin-releasing hormone agonist or human chorionic gonadotropin for final oocyte... 22074696 - Longitudinal changes in pon1 activities, pon1 phenotype distribution and oxidative stat... 14995836 - Search for extraterrestrial point sources of neutrinos with amanda-ii. |
Publication Detail:
|
Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-26 |
Journal Detail:
|
Title: Fertility and sterility Volume: 94 ISSN: 1556-5653 ISO Abbreviation: Fertil. Steril. Publication Date: 2010 Nov |
Date Detail:
|
Created Date: 2010-10-27 Completed Date: 2010-12-08 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: United States |
Other Details:
|
Languages: eng Pagination: 2078-82 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved. |
Affiliation:
|
New York University Fertility Center, New York University School of Medicine, New York, New York 10021, USA. nnoyes01@gmail.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Cells, Cultured Cryopreservation / methods* Embryo Transfer Female Humans Meiosis / physiology Mitotic Spindle Apparatus / physiology* Oocytes* Pregnancy Pregnancy Rate Retrospective Studies Temperature Time Factors Treatment Outcome |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cyclooxygenase-2 in testes of infertile men: evidence for the induction of prostaglandin synthesis b...
Next Document: Accurate single cell 24 chromosome aneuploidy screening using whole genome amplification and single ...