Document Detail

Oocyte cryopreservation outcomes including pre-cryopreservation and post-thaw meiotic spindle evaluation following slow cooling and vitrification of human oocytes.
MedLine Citation:
PMID:  20188356     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To report our oocyte cryopreservation (OC) outcomes including meiotic spindle (MS) evaluation of metaphase II (MII) oocytes destined for OC and thaw.
DESIGN: Retrospective.
SETTING: University-based infertility center.
PATIENT(S): Women attempting pregnancy using cryopreserved oocytes.
INTERVENTION(S): OC, MS evaluation.
MAIN OUTCOME MEASURE(S): Survival, two pronuclear (2PN) fertilization, achieving embryo quality suitable for transfer or refreezing, blastocyst formation.
RESULT(S): Thirty-two OC-thaw cycles resulted in 20 pregnancies, 18 either ongoing or delivered. In 26 cycles, MS evaluation was performed: 262/303 (86%) thawed/recovered oocytes survived, 218/262 (83%) achieved 2PN fertilization, 133/218 (61%) became suitable for day-3 and 122/218 (56%) for day-5 transfer. In total, 58 embryos were transferred resulting in a 62% pregnancy and a 41% implantation rate. Of oocytes evaluated before cryopreservation, 247 (82%) were spindle-positive; 96% of these were also spindle-positive after thawing. Blastocyst formation and suitability for day-5 transfer was achieved more often if a post-thaw spindle was visualized. Of all slow-cooled and vitrified oocytes, a higher percentage of those slow-cooled achieved 2PN fertilization and usability. MS evaluation of oocytes cryopreserved by either method was associated with similar outcomes.
CONCLUSION(S): OC outcomes are improving. An MS was almost always exhibited both before cryopreservation and after thawing, suggesting that, with appropriate technique, OC presents minimal harm to the MII oocyte. A meiotic spindle evaluation might help to further OC technology.
Nicole Noyes; Jaime Knopman; Patty Labella; Caroline McCaffrey; Melicia Clark-Williams; Jamie Grifo
Related Documents :
20886366 - Successful live birth from vitrified oocytes after 5 years of cryopreservation.
2442036 - Two years' experience of ultrasound-directed oocyte retrieval.
2508776 - In vitro fertilization and embryo transfer in the rhesus monkey.
19171336 - Gonadotropin-releasing hormone agonist or human chorionic gonadotropin for final oocyte...
22074696 - Longitudinal changes in pon1 activities, pon1 phenotype distribution and oxidative stat...
14995836 - Search for extraterrestrial point sources of neutrinos with amanda-ii.
Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-26
Journal Detail:
Title:  Fertility and sterility     Volume:  94     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-27     Completed Date:  2010-12-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2078-82     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
New York University Fertility Center, New York University School of Medicine, New York, New York 10021, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cells, Cultured
Cryopreservation / methods*
Embryo Transfer
Meiosis / physiology
Mitotic Spindle Apparatus / physiology*
Pregnancy Rate
Retrospective Studies
Time Factors
Treatment Outcome

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Cyclooxygenase-2 in testes of infertile men: evidence for the induction of prostaglandin synthesis b...
Next Document:  Accurate single cell 24 chromosome aneuploidy screening using whole genome amplification and single ...