Document Detail


Oocyte competence in repeat-breeder heifers: effects of an optimized ovum pick-up schedule on expression of oestrus, follicular development and fertility.
MedLine Citation:
PMID:  12895408     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Repeat-breeder heifers (RBH) have been shown to present reproductive perturbations during spontaneous cyclicity, which affects oestrus and ovulation. Some of these disturbances (e.g. deviating hormone patterns) are also present during and after cycles of twice-weekly ovum pick-up (o.p.u.), performed according to an optimized schedule allowing normal oestrous cyclicity. In the present study, the effects of o.p.u. on oocyte competence in in vitro maturation (i.v.m.) and in vitro fertilization (i.v.f.) have been evaluated, as were the effects on expression of oestrus and fertility in five RBH (> or =4 artificial inseminations) and five virgin heifers (VH controls). In total, 269 RBH and 174 VH oocytes were scored for quality prior to i.v.m. and i.v.f. The number of follicles available for puncture was higher in RBH, but the oocyte recovery rate after o.p.u. was lower in RBH compared with VH controls and the recovered RBH oocytes were of lower quality, as judged by their appearance at retrieval. Confocal laser scanning and transmission electron microscopy of immature oocytes did not reveal any differences between RBH and VH control oocytes with respect to nuclear and mitochondrial status. However, after i.v.m., the cytoplasmic spatial reorganization of mitochondria and cortical granules was less advanced in RBH, which could contribute to the subfertility that defines the syndrome. Cleavage rates after i.v.f. were similar in RBH and VH controls. Subsequent to the o.p.u. period, in vivo fertility after controlled artificial insemination was comparable with field fertility rates in both RBH and VH.
Authors:
Renée Båge; Sudsaijai Petyim; Birgitta Larsson; Triin Hallap; Ann-Sofi Bergqvist; Hans Gustafsson; Heriberto Rodríguez-Martínez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction, fertility, and development     Volume:  15     ISSN:  1031-3613     ISO Abbreviation:  Reprod. Fertil. Dev.     Publication Date:  2003  
Date Detail:
Created Date:  2003-08-04     Completed Date:  2005-03-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8907465     Medline TA:  Reprod Fertil Dev     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  115-23     Citation Subset:  IM    
Affiliation:
Centre for Reproductive Biology in Uppsala (CRU), Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, PO Box 7039, SE-750 07 Uppsala, Sweden. renee.bage@og.slu.se
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MeSH Terms
Descriptor/Qualifier:
Animals
Breeding
Cattle / physiology*
Cell Nucleus / ultrastructure
Cells, Cultured
Cleavage Stage, Ovum
Cytoplasm / ultrastructure
Estrus*
Female
Fertility*
Fertilization in Vitro / veterinary
Insemination, Artificial / veterinary
Microscopy, Confocal
Microscopy, Electron
Mitochondria / ultrastructure
Oocytes / physiology*,  ultrastructure
Ovarian Follicle* / physiology*
Progesterone / blood
Tissue and Organ Harvesting / methods,  veterinary*
Chemical
Reg. No./Substance:
57-83-0/Progesterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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