| Oocyte activation and passage through the metaphase/anaphase transition of the meiotic cell cycle is blocked in clams by inhibitors of HMG-CoA reductase activity. | |
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MedLine Citation:
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PMID: 7896878 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell cycle progression for postembryonic cells requires the activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-R), the enzyme which catalyzes the production of the isoprenoid precursor, mevalonate. In this study, we examine the requirements of HMG-R activity for cell cycle progression during the meiotic and early mitotic divisions using oocytes and dividing embryos from the surf clam, Spisula solidissima. Using two different inhibitors of HMG-R, we find that the activity of this enzyme appears to be required at three distinct points of the cell cycle during meiosis. Depending on the stage at which these inhibitors are added to synchronous clam cultures, a reversible cell cycle block is triggered at the time of activation or at metaphase of either meiosis I or II, whereas there is not block to the mitotic cell cycle. Inhibition of HMG-R activity in activated oocytes does not affect the transient activation of p42MAPK but results in a block at metaphase of meiosis I that is accompanied by the stabilization of cyclins A and B and p34cdc2 kinase activity. Our results suggest that metabolites from the mevalonate biosynthetic pathway can act to influence the process of activation, as well as the events later in the cell cycle that lead to cyclin proteolysis and the exit from M phase during clam meiosis. |
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Authors:
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J E Turner; C G Minkoff; K H Martin; R Misra; K I Swenson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of cell biology Volume: 128 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1995 Mar |
Date Detail:
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Created Date: 1995-04-24 Completed Date: 1995-04-24 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1145-62 Citation Subset: IM |
Affiliation:
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Department of Molecular Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bivalvia / cytology* Cell Cycle / drug effects Cyclins / metabolism Female Hydroxymethylglutaryl CoA Reductases / metabolism Hydroxymethylglutaryl-CoA Reductase Inhibitors* Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent Lovastatin / pharmacology Meiosis / drug effects Microtubules / drug effects, metabolism Oocytes / cytology* |
| Chemical | |
Reg. No./Substance:
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0/Cyclins; 0/Hydroxymethylglutaryl-CoA Reductase Inhibitors; 75330-75-5/Lovastatin; EC 1.1.1.-/Hydroxymethylglutaryl CoA Reductases; EC 1.1.1.34/Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 2 Month: 3 Year: 1995 Volume: 128 Issue: 6 First Page: 1145 Last Page: 1162 ID: 2120409 Publisher Id: 95204534 PubMed Id: 7896878 |
| Oocyte activation and passage through the metaphase/anaphase transition of the meiotic cell cycle is blocked in clams by inhibitors of HMG-CoA reductase activity | |
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