Document Detail

Ontogeny of expression of insulin-like growth factor (IGF) and IGF binding protein mRNAs in the guinea-pig placenta and uterus.
MedLine Citation:
PMID:  10329358     Owner:  NLM     Status:  MEDLINE    
To determine the temporal and spatial distribution of insulin-like growth factor (IGF) and its family of binding proteins (IGFBPs), guinea-pig yolk sac and chorioallantoic placentae were collected at 15, 20, 25, 29, 44-45, 55 and 65-66 days of gestation. Messenger RNAs for IGF I, IGF II and IGFBP 1-6 were identified in tissue sections by in situ hybridization, using 35S-cRNA probes. Epithelial and mesenchymal cell types were identified by immunohistochemistry for cytokeratin and vimentin, respectively. At 15 days of gestation, IGF-II mRNA was expressed in ectoplacental mesoderm, cytotrophoblasts and syncytiotrophoblast, and IGFBP-5 mRNA was detected in the syncytiotrophoblast. In the mid-gestation placenta, IGFBP-5 mRNA was expressed in the marginal and interlobular syncytium and IGF-II mRNA in the labyrinth. Near term, when expansion of the labyrinth was complete, IGFBP-5 mRNA was coexpressed with IGF-II mRNA in the marginal and interlobular syncytium. These observations suggest that interaction between IGF-II and IGFBP-5 plays a role in the vascularization of the placenta by fetal vessels. IGF-II mRNA was not expressed in the maternal tissues at any gestational age. IGFBP-2, -3 and -5 mRNAs were expressed in the endometrial stroma at 7-12 days of gestation but, following establishment of the placenta, IGFBP mRNAs were more abundant in the myometrium than in the decidua. IGF-II mRNA was detected in trophoblasts invading the walls of maternal vessels, and the endothelium of the preplacental vessels expressed IGFBP-4 mRNA, while IGFBP-2 and IGFBP-5 mRNAs were present in the tunica media of mesometrial arteries that had not been invaded by trophoblast. These findings suggest that IGF-II produced by the trophoblast acts in an autocrine and/or paracrine fashion to promote trophoblast invasion and that this process is modulated by interaction with IGFBPs present in maternal tissues.
V K Han; A M Carter; S Chandarana; B Tanswell; K Thompson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Placenta     Volume:  20     ISSN:  0143-4004     ISO Abbreviation:  Placenta     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-06-25     Completed Date:  1999-06-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  361-77     Citation Subset:  IM    
Department of Paediatrics, Lawson Research Institute, University of Western Ontario, London, Canada.
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MeSH Terms
Decidua / chemistry
Gene Expression*
Gestational Age
Guinea Pigs
In Situ Hybridization
Insulin-Like Growth Factor Binding Proteins / genetics*
Insulin-Like Growth Factor I / genetics*
Insulin-Like Growth Factor II / genetics*
Myometrium / chemistry
Placenta / chemistry*
RNA, Messenger / analysis
Uterus / chemistry*
Yolk Sac / chemistry
Reg. No./Substance:
0/Insulin-Like Growth Factor Binding Proteins; 0/RNA, Messenger; 67763-96-6/Insulin-Like Growth Factor I; 67763-97-7/Insulin-Like Growth Factor II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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