| Ontogeny of Ro hYRNAs in human heart. | |
| | |
MedLine Citation:
|
PMID: 10229139 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The Ro complex is composed of three ribonucleoproteins of 60, 54, and 52 kDa. The Ro60 is associated with the 1-5 hYRNAs. Autoantibodies against Ro are found in sera from lupus patients. Lupus frequently presents in female patients during childbearing years and transplacental passage of maternal anti-Ro/La autoantibodies has been implicated in triggering neonatal lupus and congenital heart block (CHB). Since expression of Ro ribonucleoproteins occurs before the onset of CHB, epitope spreading of Ro hYRNAs could arise during cardiac ontogeny, thus maternal autoantibodies would damage a previously developed AV node. To explore the cardiac ontogeny of Ro hYRNAs, embryonic and adult tissues were obtained from legal autopsies. In situ hybridization using oligonucleotides for hY1, hY3, hY4, hY5, and Ro60 was performed on sections of cardiac tissue; their corresponding cDNAs were amplified by RT-PCR. The principal results were as follows: expression of hY4/5RNAs was greater in fetal tissues from 8-12 weeks of development. The subcellular distribution of hYRNAs in embryonic tissues was nucleocytoplasmic, whereas in the adult heart it was cytoplasmic. In conclusion, hYRNAs expression occurs during early cardiac development. |
| | |
Authors:
|
S Fraire-Velázquez; R Herrera-Esparza; R Villalobos-Hurtado; E Avalos-Díaz |
Related Documents
:
|
16836679 - Bidirectional ventricular tachycardia with alternating right and left bundle branch blo... 616069 - The effect of mexiletine on the electrophysical properties of the intact human heart. 10556749 - Comparative study of different syringes in positive aspiration during inferior alveolar... 19632639 - Paroxysmal atrioventricular block. 9583449 - Recovery from complete atrioventricular block caused by idiopathic giant cell myocardit... 16969859 - Pathogenesis of cardiac conduction disorders in children genetic and histopathologic as... 11580109 - Uncoupling and recoupling of autonomic regulation of the heart beat in pediatric septic... 22368129 - Congenital cardiac anomalies: prenatal readings versus neonatal outcomes. 18566669 - 'no-reflow' after acute myocardial infarction: direct visualisation of microvascular ob... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Scandinavian journal of rheumatology Volume: 28 ISSN: 0300-9742 ISO Abbreviation: Scand. J. Rheumatol. Publication Date: 1999 |
Date Detail:
|
Created Date: 1999-05-13 Completed Date: 1999-05-13 Revised Date: 2007-05-11 |
Medline Journal Info:
|
Nlm Unique ID: 0321213 Medline TA: Scand J Rheumatol Country: NORWAY |
Other Details:
|
Languages: eng Pagination: 100-5 Citation Subset: IM |
Affiliation:
|
Department of Molecular Biology and Immunotechnology, CBE, Universidad Autónoma de Zacatecas, México. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Atrioventricular Node / chemistry*, embryology Autoantigens / genetics* Fetus / chemistry Gene Expression Regulation, Developmental Humans In Situ Hybridization Oligonucleotide Probes RNA, Ribosomal, 5S / analysis* RNA, Small Cytoplasmic* Reverse Transcriptase Polymerase Chain Reaction Ribonucleoproteins / genetics* |
| Chemical | |
Reg. No./Substance:
|
0/Autoantigens; 0/Oligonucleotide Probes; 0/RNA, Ribosomal, 5S; 0/RNA, Small Cytoplasmic; 0/Ribonucleoproteins; 0/SS-A antigen; 0/TROVE2 protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Circulating myeloperoxidase and anti-myeloperoxidase antibody in patients with vasculitis.
Next Document: T(H)1 cytokines are produced in labial salivary glands in Sjögren's syndrome, but also in healthy i...