| Ontogenic development of prolactin immunoreactive neurons in the rat lateral hypothalamus. | |
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MedLine Citation:
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PMID: 10102677 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present study investigated the ontogenic expression of a prolactin-like substance (oPRL-ir) in rat hypothalamus from embryonic day (E) 17 to postnatal day (P) 29. By immunocytochemistry, the oPRL-ir peptide was only detected from P3. As in adults, labeled neurons were found exclusively in the lateral hypothalamic area. By in situ hybridization, with a cocktail of oligonucleotides complementary to the PRL mRNA, no labeling was observed in the hypothalamus, although dense labeling was obtained over the pituitary. With reverse-transcriptase polymerase chain reaction, a 408 bp band, presumably corresponding to an oPRL mRNA, was detected from PO in the LHA, but also in other brain regions. These results suggest that the oPRL-ir neurons do not contain oPRL. The nature of the oPRL-ir peptide is still unknown, but its late onset of expression may be related to its putative involvement in feeding behavior. |
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Authors:
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S Grillon; C Colard; P Y Risold; D Fellmann; B Griffond |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Neuropeptides Volume: 32 ISSN: 0143-4179 ISO Abbreviation: Neuropeptides Publication Date: 1998 Aug |
Date Detail:
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Created Date: 1999-05-19 Completed Date: 1999-05-19 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8103156 Medline TA: Neuropeptides Country: SCOTLAND |
Other Details:
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Languages: eng Pagination: 327-32 Citation Subset: IM |
Affiliation:
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CNRS UPRESA 6025 and FR 51, Laboratoire d'Histologie-Embryologie-Cytogénétique, Institut d'Etudes et de Transfert de Gènes, Faculté de Médecine, Besançon, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Female Hypothalamus / chemistry, embryology* Immunohistochemistry In Situ Hybridization Male Pregnancy Prolactin / analysis*, genetics* RNA, Messenger / analysis* Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 9002-62-4/Prolactin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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