Document Detail

Ontogenic development of prolactin immunoreactive neurons in the rat lateral hypothalamus.
MedLine Citation:
PMID:  10102677     Owner:  NLM     Status:  MEDLINE    
The present study investigated the ontogenic expression of a prolactin-like substance (oPRL-ir) in rat hypothalamus from embryonic day (E) 17 to postnatal day (P) 29. By immunocytochemistry, the oPRL-ir peptide was only detected from P3. As in adults, labeled neurons were found exclusively in the lateral hypothalamic area. By in situ hybridization, with a cocktail of oligonucleotides complementary to the PRL mRNA, no labeling was observed in the hypothalamus, although dense labeling was obtained over the pituitary. With reverse-transcriptase polymerase chain reaction, a 408 bp band, presumably corresponding to an oPRL mRNA, was detected from PO in the LHA, but also in other brain regions. These results suggest that the oPRL-ir neurons do not contain oPRL. The nature of the oPRL-ir peptide is still unknown, but its late onset of expression may be related to its putative involvement in feeding behavior.
S Grillon; C Colard; P Y Risold; D Fellmann; B Griffond
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neuropeptides     Volume:  32     ISSN:  0143-4179     ISO Abbreviation:  Neuropeptides     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1999-05-19     Completed Date:  1999-05-19     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8103156     Medline TA:  Neuropeptides     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  327-32     Citation Subset:  IM    
CNRS UPRESA 6025 and FR 51, Laboratoire d'Histologie-Embryologie-Cytogénétique, Institut d'Etudes et de Transfert de Gènes, Faculté de Médecine, Besançon, France.
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MeSH Terms
Hypothalamus / chemistry,  embryology*
In Situ Hybridization
Prolactin / analysis*,  genetics*
RNA, Messenger / analysis*
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/RNA, Messenger; 9002-62-4/Prolactin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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