| Ontogenesis of lipids in chick embryo retina. | |
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MedLine Citation:
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PMID: 8950509 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: The effects of embryonic development on lipid composition in the retina were studied in 7, 11, 15, and 18-day-old chick embryos and newly hatched chicks. METHODS: The proportions of phospholipids, free and esterified cholesterol, diacylglycerides, and free fatty acids were determined using the Iatroscan TLC/FID procedure. Gas chromatography and mass spectrometry were used to determine the fatty acid composition. RESULTS: The major phospholipid species were phosphatidylethanolamine, phosphatidylcholine, phosphatidylserine, phosphatidylinositol, lysophosphatidylcholine, and sphingomyelin. Concentrations of the analyzed components have been related to the chronology of concrete stages of retinal development. The fatty acid composition of the total lipids, (n-6):(n-3) and saturated: unsaturated fatty acid ratios, and other parameters are reported. The proportions of total saturated and total monounsaturated fatty acids decreased very little from day 7 to hatching, whereas total polyunsaturated fatty acids nearly doubled over the same period. The increase in C18:2(n-6) from day 11 onwards was not followed by a similar increase in C20:4(n-6), hence the C20:4 to C18:2 ratio decreased with age. CONCLUSIONS: The cholesterol:phospholipid ratio decreased from day 7 to day 15 and increased from day 15 to hatching. High proportions of esterified cholesterol, very probably originating in the retinal pigment epithelium, were also recorded. Total saturated and monounsaturated fatty acids decreased, while polyunsaturated fatty acids increased during the period of initial retinal growth. |
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Authors:
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V Ruíz-Gutiérrez; M Dorado; L S Palazón; A R Burgos |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Current eye research Volume: 15 ISSN: 0271-3683 ISO Abbreviation: Curr. Eye Res. Publication Date: 1996 Nov |
Date Detail:
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Created Date: 1997-02-27 Completed Date: 1997-02-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8104312 Medline TA: Curr Eye Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1138-43 Citation Subset: IM |
Affiliation:
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Instituto de la Grasa, CSIC, Sevilla, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chick Embryo Cholesterol / metabolism Chromatography, Gas Fatty Acids / metabolism Lipid Metabolism* Mass Spectrometry Phospholipids / metabolism Retina / embryology*, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Phospholipids; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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