Document Detail


Only transient increase of vascular growth factors and microvascular density after percutaneous myocardial laser.
MedLine Citation:
PMID:  15492594     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: We tested the hypothesis that percutaneous myocardial laser may stimulate microvascular growth in areas surrounding the laser channels. METHODS: We conducted a study of 24 domestic pigs, which underwent percutaneous myocardial laser to left ventricular myocardium using holmium:YAG laser. The pigs were sacrificed in groups of four after one day, 3-4 days, one week, three weeks and six weeks. Frozen sections from both normal and treated myocardium were prepared for immunofluorescence microscopy and stained with antibodies against von Willebrand factor, vascular endothelial growth factor (VEGF) and Extra Domain-A cellular fibronectin (ED-AcFN). Microvascular density (MVD) and vascular area (VA) were determined in sections stained with antibodies against von Willebrand factor VIII using a digitised image analysis system. When determined in laser treated areas, channel core remnants were excluded from analysis. RESULTS: Within the laser channel remnants and in the tissue closely surrounding these, expression of VEGF and ED-AcFN increased significantly after treatment at one, 3-4, and seven days and decreased to normal at three and six weeks. Expression of ED-AcFN was detected adjacent to endothelial cells of microvessels. The original laser channels were rapidly invaded by granulation tissue. There was no sign of recanalization at any stage during the six weeks. Morphometric analysis showed no increase in MVD and VA in the myocardium surrounding the laser channels. CONCLUSION: An increase of VEGF and ED-AcFN after myocardial laser is transient and is not associated with increase of MVD or VA in myocardium not involving laser channel remnants.
Authors:
Mohamed Salem; Svein Rotevatn; Reidar J Pettersen; Karel Kuiper; Thorvald Saetersdal; Jan Erik Nordrehaug
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Coronary artery disease     Volume:  15     ISSN:  0954-6928     ISO Abbreviation:  Coron. Artery Dis.     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-10-19     Completed Date:  2005-06-16     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9011445     Medline TA:  Coron Artery Dis     Country:  England    
Other Details:
Languages:  eng     Pagination:  441-8     Citation Subset:  IM    
Affiliation:
Department of Heart Disease, Haukeland University Hospital, Bergen, Norway.
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MeSH Terms
Descriptor/Qualifier:
Animals
Coronary Vessels*
Female
Laser Therapy*
Male
Microcirculation
Myocardial Revascularization* / methods
Myocardium / chemistry*
Swine
Time Factors
Vascular Endothelial Growth Factors / analysis*
Chemical
Reg. No./Substance:
0/Vascular Endothelial Growth Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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