| Only one of the five Ralstonia solanacearum long-chain 3-ketoacyl-ACP synthase homologues functions in fatty acid synthesis. | |
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MedLine Citation:
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PMID: 22194290 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Ralstonia solanacearum, a major phytopathogenic bacterium, causes a bacterial wilt disease in diverse plants. Although fatty acid analyses of R. solanacearum showed that they contain primarily palmitic (C16:0), palmitoleic (C16: 1) and cis-vaccenic (C18: 1) acid, little is known of R. solanacearum fatty acid synthesis. The R. solanacearum GMI1000 genome is unusual in that it contains four genes (fabF1, fabF2, fabF3 and fabF4) annotated as encoding 3-ketoacyl-acyl carrier protein synthase II homologues and one gene (fabB) annotated as encoding 3-ketoacyl-acyl carrier protein synthase I. We have analyzed this puzzling apparent redundancy and found that only one of these genes, fabF1, encoded a long chain 3-ketoacyl-acyl carrier protein synthase whereas the other homologues did not play roles in R. solanacearum fatty acid synthesis. Mutant strains lacking fabF1 are nonviable and thus FabF1 is an essential for R. solanacearum fatty acid biosynthesis. Moreover, R. solanacearum FabF1 has both the activities of 3-ketoacyl-acyl carrier protein synthase II and 3-ketoacyl-acyl carrier protein synthase I. |
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Authors:
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Juanli Cheng; Jincheng Ma; Jinshui Lin; Zhen-Chuan Fan; John E Cronan; Haihong Wang |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-22 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: - ISSN: 1098-5336 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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College of Life Sciences, South China Agricultural University, Guangzhou 510642, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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