| Online monitoring of oxygen in spinner flasks. | |
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MedLine Citation:
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PMID: 19148583 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We show the application of a novel optical on-line sensor fixed in spinner flasks for the online monitoring of dissolved O(2) concentrations during mammalian cell growth. Using this sensor that requires only minute changes to the flask to be made, we could determine the volumetric O(2) transfer coefficient as well as O(2) consumption rates. Under normal growth conditions the cells did not undergo O(2) limitation. Also, the transfer of O(2) from the atmosphere to the spinner flasks is influenced by the use of screw caps. The on-line measurement was further applied to determine the O(2) uptake rates which can then be used to monitor the metabolic state of the cells and also for online process monitoring. |
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Authors:
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Rahul Ravi Deshpande; Elmar Heinzle |
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Publication Detail:
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Type: Journal Article Date: 2009-01-16 |
Journal Detail:
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Title: Biotechnology letters Volume: 31 ISSN: 1573-6776 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-04-10 Completed Date: 2009-05-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 665-9 Citation Subset: IM |
Affiliation:
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Biochemical Engineering Institute, Saarland University, Campus A1.5, 66123, Saarbrücken, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Culture Techniques
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methods Culture Media / chemistry* Oxygen / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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