| Online monitoring of cell metabolism for studying pharmacodynamic effects. | |
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MedLine Citation:
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PMID: 17320130 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To characterize modes of action of substances and their cytotoxic effects Bionas GmbH has developed a new screening system to allow the continuous recording of how an active substance can act (Bionas 2500 analyzing system). In the pharmaceutical industry it is important to acquire as much information as possible about the metabolic effects of an active substance. Most classical pre-clinical studies are very expensive and time-consuming. Often they are so-called end-point tests which require many individual tests before approximate statements can be made about how an effect takes its course. With the Bionas 2500 analyzing system metabolically relevant data including oxygen consumption, acidification rate and the adhesion (cell impedance) of cells can be measured in parallel, online and label-free. Using e.g. ion-sensitive field effect-transistors (ISFET) and electrode structures it is possible to observe metabolic parameters non-invasively and continuously over longer periods of time. The system has already been established for several cell models, cell lines as well as primary cells. It also offers the advantage that regenerative effects can be observed during the same test run. |
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Authors:
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Elke Thedinga; Axel Kob; Heiko Holst; Andreas Keuer; Sabine Drechsler; Ricarda Niendorf; Werner Baumann; Ingo Freund; Mirko Lehmann; Ralf Ehret |
Publication Detail:
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Type: Journal Article Date: 2007-01-03 |
Journal Detail:
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Title: Toxicology and applied pharmacology Volume: 220 ISSN: 0041-008X ISO Abbreviation: Toxicol. Appl. Pharmacol. Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-03-23 Completed Date: 2007-05-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0416575 Medline TA: Toxicol Appl Pharmacol Country: United States |
Other Details:
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Languages: eng Pagination: 33-44 Citation Subset: IM |
Affiliation:
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Bionas GmbH, Friedrich-Barnewitz-Str. 3, 18119 Rostock, Germany. elke.thedinga@bionas.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Animals Biosensing Techniques / instrumentation* Cell Adhesion* Cell Line Cycloheximide / pharmacology Humans Hydrogen-Ion Concentration* Monitoring, Physiologic / instrumentation* Oxygen Consumption* |
| Chemical | |
Reg. No./Substance:
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56-65-5/Adenosine Triphosphate; 66-81-9/Cycloheximide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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