Document Detail

Ongoing pregnancies after vitrification of human oocytes using a combined solution of ethylene glycol and dimethyl sulfoxide.
MedLine Citation:
PMID:  16963044     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To evaluate a vitrification solution using a mixture of two cryprotectant agents, dimethyl sulfoxide and ethylyne glycol plus sucrose, on the survival of human oocytes. DESIGN: Clinical study of cryopreservation of human metaphase II (MII) oocytes by vitrification. SETTING: University-affiliated IVF center. PATIENT(S): Infertile couples who agreed to have their surplus oocytes vitrified during the fresh IVF cycle. INTERVENTION(S): Vitrification of surplus oocytes subsequently used in the next cycle and assisted fertilization by intracytoplasmic sperm injection. MAIN OUTCOME MEASURE(S): Morphologic survival and normal fertilization, embryo development, and clinical outcome. RESULT(S): A total of 53 surplus MII oocytes from 6 patients were vitrified, of which 24 were thawed, resulting in 18 which survived morphologically (75%). Following insemination, 14 of the 18 surviving eggs were fertilized (77.7%). All zygotes developed into viable embryos that were replaced into each patient's uterus, resulting in two healthy pregnancies: one singleton and one twin. The pregnancies were ongoing. CONCLUSION(S): Cryopreservation of human MII oocytes by vitrification appears to be a promising procedure, though to assure optimal effectiveness of this protocol further studies should be undertaken.
Helmy Selman; Antonio Angelini; Nicoletta Barnocchi; Gian Francesco Brusco; Arianna Pacchiarotti; Cesare Aragona
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Publication Detail:
Type:  Journal Article     Date:  2006-09-11
Journal Detail:
Title:  Fertility and sterility     Volume:  86     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-10-09     Completed Date:  2006-11-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  997-1000     Citation Subset:  IM    
Centro Sterilità e Fecondazione Assistita Policlinico di Perugia, University of Perugia, Perugia, Italy.
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MeSH Terms
Cell Survival / drug effects
Cells, Cultured
Cryopreservation / methods*
Cryoprotective Agents / administration & dosage
Dimethyl Sulfoxide / administration & dosage*
Drug Combinations
Ethylene Glycol / administration & dosage*
Fertilization in Vitro / methods*
Infertility / therapy*
Oocytes / cytology,  drug effects*,  transplantation*
Pregnancy Outcome
Treatment Outcome
Reg. No./Substance:
0/Cryoprotective Agents; 0/Drug Combinations; 107-21-1/Ethylene Glycol; 67-68-5/Dimethyl Sulfoxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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