Document Detail


One-step purification of bacterial lipid macroamphiphiles by hydrophobic interaction chromatography.
MedLine Citation:
PMID:  1862938     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bacterial lipid macroamphiphiles extracted with phenol/water can be purified in one step by hydrophobic interaction chromatography. Lipids and the major part of protein are separated from macroamphiphiles during phenol/water extraction. Coextracted nucleic acids, polysaccharides, and residual protein are effectively removed by column chromatography on octyl-Sepharose whereby macroamphiphiles are primarily adsorbed and later eluted with a buffered propanol gradient. The procedure is applicable to macroamphiphiles with various lipid structures as was demonstrated using the diacylglycerol-containing lipoglycan of Micrococcus luteus, the lipid A-containing lipopolysaccharide of Salmonella typhimurium, and the diglyceryl tetraether lipoglycans of Thermoplasma acidophilum and Thermoplasma volcanicum. On elution from octyl-Sepharose, separation into molecular species of different compositions was observed with the lipopolysaccharide of S. typhimurium and the lipoglycan of T. volcanicum. It was also shown that, after phenol/water extraction, membrane lipids are completely recoverable from the phenol layer, which makes it possible to isolate lipids along with macroamphiphiles from the same sample of bacteria.
Authors:
W Fischer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical biochemistry     Volume:  194     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1991 May 
Date Detail:
Created Date:  1991-08-30     Completed Date:  1991-08-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  353-8     Citation Subset:  IM    
Affiliation:
Institut für Biochemie, Medizinische Fakultät, Universität Erlangen-Nürnberg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Agarose / methods*
Ethers / isolation & purification
Lipopolysaccharides / isolation & purification*
Micrococcus / analysis
Phenols
Salmonella typhimurium / analysis
Solubility
Thermoplasma / analysis
Water
Chemical
Reg. No./Substance:
0/Ethers; 0/Lipopolysaccharides; 0/Phenols; 0/lipomannan; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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