| One-step extraction of human erythrocyte lipids allowing rapid determination of fatty acid composition. | |
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MedLine Citation:
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PMID: 2817399 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A one-step extraction procedure to directly quantify cholesterol, phospholipids, and fatty acids in red blood cell membranes has been developed. The method uses a single solvent, isopropanol, which extracts lipids and allows the rapid formation of isopropylic esters of fatty acids by acid catalysis. The efficiency of this new technique has been confirmed by comparing yields of cholesterol and total and individual phospholipids with yields obtained following conventional extraction procedures. Moreover, in comparison to the formation of methyl esters, we demonstrate that directly obtained isopropylic esters immediately allow the quantitative determination of fatty acids, without involving the hydrolytic degradation of fatty acids and the oxidation of unsaturated fatty acids. |
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Authors:
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E Peuchant; R Wolff; C Salles; R Jensen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Analytical biochemistry Volume: 181 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1989 Sep |
Date Detail:
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Created Date: 1989-11-27 Completed Date: 1989-11-27 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 341-4 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Saint-André Hospital, Bordeaux, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chloroform Chromatography, Gas Chromatography, Thin Layer Erythrocyte Membrane / analysis* Fatty Acids / blood* Humans Membrane Lipids / blood* Methanol Solvents |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Membrane Lipids; 0/Solvents; 67-56-1/Methanol; 67-66-3/Chloroform |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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