Document Detail


One-step extraction of human erythrocyte lipids allowing rapid determination of fatty acid composition.
MedLine Citation:
PMID:  2817399     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A one-step extraction procedure to directly quantify cholesterol, phospholipids, and fatty acids in red blood cell membranes has been developed. The method uses a single solvent, isopropanol, which extracts lipids and allows the rapid formation of isopropylic esters of fatty acids by acid catalysis. The efficiency of this new technique has been confirmed by comparing yields of cholesterol and total and individual phospholipids with yields obtained following conventional extraction procedures. Moreover, in comparison to the formation of methyl esters, we demonstrate that directly obtained isopropylic esters immediately allow the quantitative determination of fatty acids, without involving the hydrolytic degradation of fatty acids and the oxidation of unsaturated fatty acids.
Authors:
E Peuchant; R Wolff; C Salles; R Jensen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  181     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1989 Sep 
Date Detail:
Created Date:  1989-11-27     Completed Date:  1989-11-27     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  341-4     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Saint-André Hospital, Bordeaux, France.
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MeSH Terms
Descriptor/Qualifier:
Chloroform
Chromatography, Gas
Chromatography, Thin Layer
Erythrocyte Membrane / analysis*
Fatty Acids / blood*
Humans
Membrane Lipids / blood*
Methanol
Solvents
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Membrane Lipids; 0/Solvents; 67-56-1/Methanol; 67-66-3/Chloroform

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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