| One-step analysis of major bile components in human bile using 1H NMR spectroscopy. | |
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MedLine Citation:
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PMID: 16981436 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human gallbladder bile dissolved in dimethylsulfoxide provides sharp and resolved signals for major bile components in 1H NMR spectra. Characteristic well-resolved marker signals that invariably appear in 1H NMR spectra of bile were identified for cholesterol (H18 methyl signal at 0.643 ppm), lipids (glycerol CH signal at 5.064 ppm), total bile acids (H18 signals in the range 0.520-0.626 ppm), total glycine conjugated bile acids (NH signal at 6.958 ppm), total taurine conjugated bile acids (NH signal at 7.646 ppm), and urea (NH2 signal near 5.48 ppm), which enabled their rapid and accurate analysis. Excellent linearity and precision of quantitative analysis was observed for all the identified bile components (R2 > 0.99 for all). The method was demonstrated on gallbladder bile from 19 patients with gallbladder diseases. Urea in bile was identified by NMR for the first time and its quantitative analysis, along with several other bile components, is presented. The majority of the bile components could be analyzed in a single step. Accurate and rapid quantification of several bile components noninvasively by using the method presented herein may have far-reaching implications in the study of bile acid metabolism and pathophysiology of various hepatobiliary and gastrointestinal diseases. |
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Authors:
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G A Nagana Gowda; B S Somashekar; Omkar B Ijare; Ajay Sharma; V K Kapoor; C L Khetrapal |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Lipids Volume: 41 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-09-19 Completed Date: 2006-11-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: United States |
Other Details:
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Languages: eng Pagination: 577-89 Citation Subset: IM |
Affiliation:
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Centre of Biomedical Magnetic Resonance, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow-226 014, India. gangcbmr@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bile
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chemistry* Cholecystitis / pathology Dimethyl Sulfoxide / pharmacology Gallbladder / pathology, secretion Gallbladder Neoplasms / pathology Humans Mass Spectrometry / methods Models, Biological Nuclear Magnetic Resonance, Biomolecular / methods* Signal Processing, Computer-Assisted Urea / analysis Water / pharmacology Xanthomatosis / pathology |
| Chemical | |
Reg. No./Substance:
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57-13-6/Urea; 67-68-5/Dimethyl Sulfoxide; 7732-18-5/Water |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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