Document Detail


One-step (18)F-labeling of peptides for positron emission tomography imaging using the SiFA methodology.
MedLine Citation:
PMID:  23037309     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Here we present a procedure to label peptides with the positron-emitting radioisotope fluorine-18 ((18)F) using the silicon-fluoride acceptor (SiFA) labeling methodology. Positron emission tomography (PET) has gained high importance in noninvasive imaging of various diseases over the past decades, and thus new specific imaging probes for PET imaging, especially those labeled with (18)F, because of the advantageous properties of this nuclide, are highly sought after. N-terminally SiFA-modified peptides can be labeled with (18)F(-) in one step at room temperature (20-25 °C) or below without forming side products, thereby producing satisfactory radiochemical yields of 46 ± 1.5% (n = 10). The degree of chemoselectivity of the (18)F-introduction, which is based on simple isotopic exchange, allows for a facile cartridge-based purification fully devoid of HPLC implementation, thereby yielding peptides with specific activities between 44.4 and 62.9 GBq μmol(-1) (1,200-1,700 Ci mmol(-1)) within 25 min.
Authors:
Carmen Wängler; Sabrina Niedermoser; Joshua Chin; Katy Orchowski; Esther Schirrmacher; Klaus Jurkschat; Liuba Iovkova-Berends; Alexey P Kostikov; Ralf Schirrmacher; Björn Wängler
Related Documents :
24753099 - Complex difference constrained compressed sensing reconstruction for accelerated prf th...
19699059 - Intracranial eeg power and metabolism in human epilepsy.
7795979 - Technical note: multimedia clinical records: results of a pilot project at the radiatio...
15134409 - Combining technologies: a computerized occlusal analysis system synchronized with a com...
19162739 - Evaluating the performance of a self-paced bci with a new movement and using a more eng...
3362069 - Quantitation of phosphate metabolites in human leg in vivo.
3568559 - A dual-frequency applied potential tomography technique: computer simulations.
11373919 - Congenital surfactant protein b deficiency--emphasis on imaging.
22678839 - Predicting consumer behavior: using novel mind-reading approaches.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-04
Journal Detail:
Title:  Nature protocols     Volume:  7     ISSN:  1750-2799     ISO Abbreviation:  Nat Protoc     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-05     Completed Date:  2013-03-19     Revised Date:  2013-03-21    
Medline Journal Info:
Nlm Unique ID:  101284307     Medline TA:  Nat Protoc     Country:  England    
Other Details:
Languages:  eng     Pagination:  1946-55     Citation Subset:  IM    
Affiliation:
Biomedical Chemistry, Medical Research Center Mannheim, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Chromatography, High Pressure Liquid
Fluorine Radioisotopes*
Isotope Labeling / methods*
Organosilicon Compounds / analysis,  chemistry
Peptides / analysis*,  chemistry
Positron-Emission Tomography / methods*
Temperature
Chemical
Reg. No./Substance:
0/Fluorine Radioisotopes; 0/Organosilicon Compounds; 0/Peptides; 0/p-(di-tert-butyl((18)F)fluorosilyl)benzaldehyde

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Using multiplex single-base extension typing to screen for mutants defective in RNA editing.
Next Document:  Synthesis of [(18)F]SiFB: a prosthetic group for direct protein radiolabeling for application in pos...