Document Detail

One-pot synthesis of aptamer functionalized silver nanoclusters for cell-type specific imaging.
MedLine Citation:
PMID:  22482827     Owner:  NLM     Status:  Publisher    
As an emerging category of fluorescent metal nanoclusters, oligonucleotide-templated silver nanoclusters (Ag NCs) have attracted a lot of interest and have showed widely applications in bio-related disciplines. However, the weak fluorescence emission and poor permeability to cell membranes tethered further intracellular applications of Ag NCs. AS1411 is an anti-proliferative G-rich phosphodiester oligonucleotide and currently an anticancer agent under Phase Ⅱclinical trials. Herein, we present a strategy to synthesize AS1411 functionalized Ag NCs with excellent fluorescence through a facile one-pot process. Confocal laser scanning microscopy and Z-axis scanning confirmed that the AS1411 functionalized Ag NCs could be internalized into MCF-7 human breast cancer cells and was able to specifically stain nuclei with red color. To our surprise, 3-[4,5-Dimethylthiazol-z-yl]-2,5-diphenyltetrazolium bromide (MTT) assay demonstrated the Ag NCs were cyto-compatible and better inhibition effect than pure AS1411 to MCF-7 human breast cancer cells. Besides, a universal design of the oligonucleotide scaffold for the synthesis of Ag NCs was extended to other aptamers, i.e. Sgc8c and Mucin 1 aptamer. Due to the facile synthesis procedure and capability of specific target recognition, this fluorescent platform will potentially broaden the applications of Ag NCs in biosensing and biological imaging.
Jingjing Li; Xiaoqin Zhong; Fangfang Cheng; Jian-Rong Zhang; Li-Ping Jiang; Jun-Jie Zhu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-7
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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