Document Detail


One-pot labeling-based capillary zone electrophoresis for separation of amino acid mixture and assay of biofluids.
MedLine Citation:
PMID:  21889643     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A fast, simple and cost-effective one-pot labeling strategy coupled with capillary zone electrophoresis was developed for the complete separation of amino acid mixture. The strategy includes two steps of reactions: Cyanuric chloride was made to react first with 7-amino-1,3-naphthalenedisulfonic acid monopotassium salt at 0°C for 10min, and then with amino acids at 55°C for 6min. The resulted products, after diluted with water, were injected into capillary zone electrophoresis system for separation. Using a running buffer of 20mM sodium tetraborate decahydrate at pH 10.1, nineteen amino acids were efficiently separated in 25min, with relative standard deviation of 0.36-1.6% and 0.96-2.1% (within and between days, respectively) for migration time and 0.030-1.6% and 0.22-2.4% (within and between days, respectively) for peak area. The proposed method has been successfully applied to the determination of free amino acids in biofluids, including human serum, urine, and saliva. The linearity of quantification was over two orders of magnitude for most amino acids, with a correlation coefficient larger than 0.999. The average recovery, determined by spiking a known amount of amino acid standards into real samples, was in a range from 91.6% to 105.9%. This method can be a noninvasive means since it could directly assay the urine and saliva samples.
Authors:
Lijuan Song; Zhenpeng Guo; Yi Chen
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Publication Detail:
Type:  Journal Article     Date:  2011-08-03
Journal Detail:
Title:  Analytica chimica acta     Volume:  703     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-09-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  257-63     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:
Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
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