Document Detail


One-pot conversion of levan prepared from Serratia levanicum NN to difructose anhydride IV by Arthrobacter nicotinovorans levan fructotransferase.
MedLine Citation:
PMID:  20159571     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The newly established difructose anhydride IV (DFA IV) production system is comprised of the effective production of levan from sucrose by Serratia levanicum NN, the conversion of the levan into DFA IV by levan fructotransferase from Arthrobacter nicotinovorans GS-9, which is highly expressed in an Escherichiacoli transformant, and a practical purification step. The chemical properties of DFA IV were also investigated.
Authors:
Hiroto Kikuchi; Hiroaki Sakurai; Taizo Nagura; Tsutomu Aritsuka; Fusao Tomita; Atsushi Yokota
Publication Detail:
Type:  Journal Article     Date:  2009-10-20
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  109     ISSN:  1347-4421     ISO Abbreviation:  J. Biosci. Bioeng.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-17     Completed Date:  2010-05-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  240-3     Citation Subset:  IM    
Copyright Information:
Copyright 2009. Published by Elsevier B.V.
Affiliation:
Research Center, Nippon Beet Sugar Mfg. Co., Ltd., 9-13, Inada-cho, Obihiro, Hokkaido 080-0831, Japan.
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MeSH Terms
Descriptor/Qualifier:
Arthrobacter / enzymology*
Disaccharides / biosynthesis*,  chemistry
Fructans / chemistry*,  metabolism*
Hexosyltransferases / chemistry*,  metabolism*
Serratia / metabolism*
Chemical
Reg. No./Substance:
0/Disaccharides; 0/Fructans; 546-42-9/difructose anhydride IV; 9013-95-0/levan; EC 2.4.1.-/Hexosyltransferases; EC 2.4.1.-/levan fructotransferase

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