| One-minute full-gradient HPLC/UV/ELSD/MS analysis to support high-throughput parallel synthesis. | |
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MedLine Citation:
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PMID: 15360216 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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High-throughput parallel synthesis of library compounds for early drug discovery requires high-throughput analytical methods to confirm synthesis, identify reaction products, and determine purity. An ultrafast 1.0-min HPLC/UV/ELSD/MS method was developed and compared to our standard 2.5- and 5.0-min methods in order to determine if the faster method was appropriate to evaluate compound synthesis and determine purity. In addition to using standard test mixtures, a 400-member library produced by high-throughput parallel synthesis was used for comparing the various methods. Mass spectrometric detection was used for compound identification, while UV and ELSD data offered purity assessment. Compared to our longer separations, chromatographic separation achieved using the 1.0-min method was sufficient for compound evaluation and purity assessment. This ultrafast 1.0-min HPLC/UV/ELSD/MS method is expected to increase analytical throughput tremendously, provide important information faster, and reduce the overall cycle time from synthesis to screening. |
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Authors:
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James N Kyranos; Heewon Lee; Wolfgang K Goetzinger; Lily Y T Li |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of combinatorial chemistry Volume: 6 ISSN: 1520-4766 ISO Abbreviation: J Comb Chem Publication Date: 2004 Sep-Oct |
Date Detail:
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Created Date: 2004-09-13 Completed Date: 2006-01-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100886263 Medline TA: J Comb Chem Country: United States |
Other Details:
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Languages: eng Pagination: 796-804 Citation Subset: - |
Affiliation:
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ArQule, Inc., 19 Presidential Way, Woburn, Massachusetts 01801, USA. jkyranos@arqule.com |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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