| One hour reperfusion is enough to assess function and infarct size with TTC staining in Langendorff rat model. | |
| | |
MedLine Citation:
|
PMID: 19466533 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: There is not general agreement concerning the optimal time of reperfusion necessary to assess myocardial function and necrosis on isolated perfused heart model. Nevertheless, the study of cardioprotection (especially, pre- and postconditioning) requires a reliable and standardized assessment of myocardial necrosis. OBJECTIVE: The objective of this study was thus to evaluate whether 1 h of reperfusion was sufficient to assess rat heart viability on Langendorff preparation. Isolated rat hearts (n = 30) underwent 40 min of global normothermic ischemia followed by 60 or 120 min Langendorff reperfusion. In each group, hearts were also randomly assigned into the 2 following sub-groups: postconditioning (PostC, consisting in 2 episodes of 30 s ischemia and 30 s reperfusion at the onset of reperfusion), and control (no intervention). Coronary flow, heart rate, dP/dt and rate-pressure-product were measured. Myocardial necrosis was assessed by TTC staining and LDH, CK release analysis. RESULTS: Our results indicated that heart function tended to slightly decrease between 60 min and 120 min reperfusion. Infarct size was identical at 60 min and 120 min reperfusion, averaging 33-34% of total LV area in controls versus 17% in PostC (p < 0.001 between control and PostC groups). Similarly, the maximum of enzymatic releases (CK and LDH) measured in coronary effluents was at 60 min of reperfusion, followed by a progressive decrease at 90 min and 120 min. As expected, postconditioning limited enzymatic releases whatever the studied time of reperfusion. CONCLUSION: In conclusion, we showed that prolonged reperfusion beyond 60 min was not useful for function assessment and did not change infarct size measurement, on Langendorff rat model of ischemia-reperfusion. |
| | |
Authors:
|
R Ferrera; S Benhabbouche; J C Bopassa; B Li; M Ovize |
Related Documents
:
|
7728993 - Increased in vivo expression and production of endothelin-1 by porcine cardiomyocytes s... 3150113 - The effects of lipoxygenase inhibitor and peptidoleukotriene antagonist on myocardial i... 18759133 - Non-invasive coronary angiography: the clinical value of multi-slice computed tomograph... |
Publication Detail:
|
Type: In Vitro; Journal Article |
Journal Detail:
|
Title: Cardiovascular drugs and therapy / sponsored by the International Society of Cardiovascular Pharmacotherapy Volume: 23 ISSN: 1573-7241 ISO Abbreviation: - Publication Date: 2009 Aug |
Date Detail:
|
Created Date: 2009-08-21 Completed Date: 2009-10-26 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8712220 Medline TA: Cardiovasc Drugs Ther Country: United States |
Other Details:
|
Languages: eng Pagination: 327-31 Citation Subset: IM |
Affiliation:
|
INSERM U886, Université de Lyon, Université Lyon 1, Lyon, F-69008, France. rene.ferrera@recherche.univ-Pyon1.fr |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Disease Models, Animal Heart Rate Ischemic Preconditioning, Myocardial / methods* Male Myocardial Reperfusion Injury / physiopathology* Myocardium / pathology Necrosis / physiopathology* Rats Rats, Wistar Staining and Labeling Tetrazolium Salts Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/Tetrazolium Salts; 902-00-1/triphenyltetrazolium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Human aortic endothelial cell response to 316L stainless steel material microstructure.
Next Document: No Article Title