Document Detail

One hour reperfusion is enough to assess function and infarct size with TTC staining in Langendorff rat model.
MedLine Citation:
PMID:  19466533     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: There is not general agreement concerning the optimal time of reperfusion necessary to assess myocardial function and necrosis on isolated perfused heart model. Nevertheless, the study of cardioprotection (especially, pre- and postconditioning) requires a reliable and standardized assessment of myocardial necrosis. OBJECTIVE: The objective of this study was thus to evaluate whether 1 h of reperfusion was sufficient to assess rat heart viability on Langendorff preparation. Isolated rat hearts (n = 30) underwent 40 min of global normothermic ischemia followed by 60 or 120 min Langendorff reperfusion. In each group, hearts were also randomly assigned into the 2 following sub-groups: postconditioning (PostC, consisting in 2 episodes of 30 s ischemia and 30 s reperfusion at the onset of reperfusion), and control (no intervention). Coronary flow, heart rate, dP/dt and rate-pressure-product were measured. Myocardial necrosis was assessed by TTC staining and LDH, CK release analysis. RESULTS: Our results indicated that heart function tended to slightly decrease between 60 min and 120 min reperfusion. Infarct size was identical at 60 min and 120 min reperfusion, averaging 33-34% of total LV area in controls versus 17% in PostC (p < 0.001 between control and PostC groups). Similarly, the maximum of enzymatic releases (CK and LDH) measured in coronary effluents was at 60 min of reperfusion, followed by a progressive decrease at 90 min and 120 min. As expected, postconditioning limited enzymatic releases whatever the studied time of reperfusion. CONCLUSION: In conclusion, we showed that prolonged reperfusion beyond 60 min was not useful for function assessment and did not change infarct size measurement, on Langendorff rat model of ischemia-reperfusion.
R Ferrera; S Benhabbouche; J C Bopassa; B Li; M Ovize
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Cardiovascular drugs and therapy / sponsored by the International Society of Cardiovascular Pharmacotherapy     Volume:  23     ISSN:  1573-7241     ISO Abbreviation:  -     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-08-21     Completed Date:  2009-10-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712220     Medline TA:  Cardiovasc Drugs Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  327-31     Citation Subset:  IM    
INSERM U886, Université de Lyon, Université Lyon 1, Lyon, F-69008, France.
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MeSH Terms
Disease Models, Animal
Heart Rate
Ischemic Preconditioning, Myocardial / methods*
Myocardial Reperfusion Injury / physiopathology*
Myocardium / pathology
Necrosis / physiopathology*
Rats, Wistar
Staining and Labeling
Tetrazolium Salts
Time Factors
Reg. No./Substance:
0/Tetrazolium Salts; 902-00-1/triphenyltetrazolium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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