| One hour of myocardial ischemia decreases the activity of the stimulatory guanine-nucleotide regulatory protein Gs. | |
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MedLine Citation:
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PMID: 2551529 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of 1 hour of myocardial ischemia on the function of the stimulatory guanine-nucleotide-binding protein Gs was examined. This study follows our recent finding that myocardial ischemia increases the density of beta-adrenoreceptors in a conscious canine model while having the opposite effect on the activity of adenylate cyclase. Coronary artery occlusion was induced in five conscious dogs and verified by measurement of blood flow using the Doppler and microsphere techniques. Alterations in the level and function of Gs were examined in sarcolemmal membranes prepared from ischemic and nonischemic regions of the left ventricle. After 1 hour of coronary artery occlusion, the functional activity of sarcolemmal Gs, as determined by reconstitution with cyc- membranes, decreased by 27 +/- 7% in the ischemic zone. Cholera toxin labeling performed in parallel with the reconstitution studies demonstrated a similar decrease of 28 +/- 7%. This was associated with decreases in basal activity and decreases in adenylate cyclase activity stimulated by GTP, GTP plus isoproterenol, sodium fluoride, and forskolin. Thus, a defect distal to the beta-adrenoreceptor occurs in the transduction of adrenergic signals to the heart as a consequence of 1 hour of ischemia. |
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Authors:
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E E Susanni; W T Manders; D R Knight; D E Vatner; S F Vatner; C J Homcy |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Circulation research Volume: 65 ISSN: 0009-7330 ISO Abbreviation: Circ. Res. Publication Date: 1989 Oct |
Date Detail:
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Created Date: 1989-11-09 Completed Date: 1989-11-09 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0047103 Medline TA: Circ Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1145-50 Citation Subset: IM; S |
Affiliation:
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Cardiac Unit, Massachusetts General Hospital, Boston. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenylate Cyclase
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metabolism Animals Cholera Toxin / diagnostic use Coronary Disease / enzymology, metabolism*, physiopathology Dogs Female GTP-Binding Proteins / metabolism* Hemodynamics Male Receptors, Adrenergic, beta / metabolism Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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HL-33065/HL/NHLBI NIH HHS; HL-37404/HL/NHLBI NIH HHS; HL-38070/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Adrenergic, beta; 9012-63-9/Cholera Toxin; EC 3.6.1.-/GTP-Binding Proteins; EC 4.6.1.1/Adenylate Cyclase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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