| One-dimensional self-assembly of mouse embryonic stem cells using an array of hydrogel microstrands. | |
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MedLine Citation:
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PMID: 21459438 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ability of embryonic stem (ES) cells to self-renew indefinitely and to differentiate into multiple cell lineages holds promise for advances in modeling disease progression, screening drugs and treating diseases. To realize these potentials, it is imperative to study self-assembly in an embryonic microenvironment, as this may increase our understanding of ES cell maintenance and differentiation. In this study, we synthesized an array of one-dimensional alginate gel microstrands and aqueous microstrands through an SU-8 filter device by means of capillary action. Furthermore, we investigated self-assembly behaviors and differentiation potentials of mouse ES cells cultured in microstrands of varying diameters. We found that microstrands with an aqueous interior facilitated high density cell culture and formed compact microtissue structures, while microstrands with gelled interiors promote smaller cell aggregate structures. In particular, we noticed that ES cells collected from one-dimensional aqueous microstrands favored the differentiation towards cell lineages of endoderm and mesoderm, whereas those from gelled microstrands preferred to differentiate into ectoderm and mesoderm lineages. In addition to providing a "liquid-like" tubular microenvironment to understand one-dimensional self-assembly process of ES cells, this alginate hydrogel microstrand system also offers an alternative way to manipulate the stem cell fate-decision using bioengineered microenvironments. |
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Authors:
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Nurazhani Abdul Raof; Michael R Padgen; Alison R Gracias; Magnus Bergkvist; Yubing Xie |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2011-04-02 |
Journal Detail:
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Title: Biomaterials Volume: 32 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-05-09 Completed Date: 2011-08-22 Revised Date: 2012-09-18 |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 4498-505 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
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College of Nanoscale Science and Engineering, University at Albany, State University of New York (SUNY), Albany, NY 12203, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alginates
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chemistry Animals Biocompatible Materials / chemistry, metabolism Cell Culture Techniques / methods* Cell Differentiation Cell Lineage Cells, Cultured Embryonic Stem Cells / cytology, physiology* Endoderm / cytology Hydrogels / chemistry* Materials Testing Mesoderm / cytology Mice Microfluidics / instrumentation*, methods* Water / chemistry |
| Grant Support | |
ID/Acronym/Agency:
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1R56DK088217-01/DK/NIDDK NIH HHS; R56 DK088217-01/DK/NIDDK NIH HHS; R56 DK088217-02/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Alginates; 0/Biocompatible Materials; 0/Hydrogels; 7732-18-5/Water |
| Comments/Corrections | |
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