Document Detail

One-dimensional self-assembly of mouse embryonic stem cells using an array of hydrogel microstrands.
MedLine Citation:
PMID:  21459438     Owner:  NLM     Status:  MEDLINE    
The ability of embryonic stem (ES) cells to self-renew indefinitely and to differentiate into multiple cell lineages holds promise for advances in modeling disease progression, screening drugs and treating diseases. To realize these potentials, it is imperative to study self-assembly in an embryonic microenvironment, as this may increase our understanding of ES cell maintenance and differentiation. In this study, we synthesized an array of one-dimensional alginate gel microstrands and aqueous microstrands through an SU-8 filter device by means of capillary action. Furthermore, we investigated self-assembly behaviors and differentiation potentials of mouse ES cells cultured in microstrands of varying diameters. We found that microstrands with an aqueous interior facilitated high density cell culture and formed compact microtissue structures, while microstrands with gelled interiors promote smaller cell aggregate structures. In particular, we noticed that ES cells collected from one-dimensional aqueous microstrands favored the differentiation towards cell lineages of endoderm and mesoderm, whereas those from gelled microstrands preferred to differentiate into ectoderm and mesoderm lineages. In addition to providing a "liquid-like" tubular microenvironment to understand one-dimensional self-assembly process of ES cells, this alginate hydrogel microstrand system also offers an alternative way to manipulate the stem cell fate-decision using bioengineered microenvironments.
Nurazhani Abdul Raof; Michael R Padgen; Alison R Gracias; Magnus Bergkvist; Yubing Xie
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-04-02
Journal Detail:
Title:  Biomaterials     Volume:  32     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-05-09     Completed Date:  2011-08-22     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  4498-505     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
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MeSH Terms
Alginates / chemistry
Biocompatible Materials / chemistry,  metabolism
Cell Culture Techniques / methods*
Cell Differentiation
Cell Lineage
Cells, Cultured
Embryonic Stem Cells / cytology,  physiology*
Endoderm / cytology
Hydrogels / chemistry*
Materials Testing
Mesoderm / cytology
Microfluidics / instrumentation*,  methods*
Water / chemistry
Grant Support
1R56DK088217-01/DK/NIDDK NIH HHS; R56 DK088217/DK/NIDDK NIH HHS; R56 DK088217-01/DK/NIDDK NIH HHS
Reg. No./Substance:
0/Alginates; 0/Biocompatible Materials; 0/Hydrogels; 059QF0KO0R/Water

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