Document Detail

One-Pot Assembly of a Hetero-Dimeric DNA Origami from Chip-Derived Staples and Double-Stranded Scaffold.
MedLine Citation:
PMID:  23281627     Owner:  NLM     Status:  Publisher    
Although structural DNA nanotechnology, and especially scaffolded DNA origami, hold great promise for bottom-up fabrication of novel nanoscale materials and devices, concerns about scalability have tempered widespread enthusiasm. Here we report a single-pot reaction where both strands of double-stranded M13-bacteriophage DNA are simultaneously folded into two distinct shapes that then hetero-dimerize with high yield. The fully addressable, two-dimensional hetero-dimer DNA origami, with twice the surface area of standard M13 origami, formed in high yield (86% of the well-formed monomers undergo dimerization). We also report the concurrent production of entire sets of staple strands by a unique, nicking strand-displacement amplification (nSDA) involving reusable surface-bound template strands that were synthesized in situ using a custom piezoelectric inkjet system. The combination of chip-based staple strand production, double-sized origami, and high-yield one-pot assembly markedly increase the useful scale of DNA origami.
Alexandria N Marchi; Ishtiaq Saaem; Jingdong Tian; Thomas H Labean
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-2
Journal Detail:
Title:  ACS nano     Volume:  -     ISSN:  1936-086X     ISO Abbreviation:  ACS Nano     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101313589     Medline TA:  ACS Nano     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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