Document Detail


One-Pot Assembly of a Hetero-Dimeric DNA Origami from Chip-Derived Staples and Double-Stranded Scaffold.
MedLine Citation:
PMID:  23281627     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Although structural DNA nanotechnology, and especially scaffolded DNA origami, hold great promise for bottom-up fabrication of novel nanoscale materials and devices, concerns about scalability have tempered widespread enthusiasm. Here we report a single-pot reaction where both strands of double-stranded M13-bacteriophage DNA are simultaneously folded into two distinct shapes that then hetero-dimerize with high yield. The fully addressable, two-dimensional hetero-dimer DNA origami, with twice the surface area of standard M13 origami, formed in high yield (86% of the well-formed monomers undergo dimerization). We also report the concurrent production of entire sets of staple strands by a unique, nicking strand-displacement amplification (nSDA) involving reusable surface-bound template strands that were synthesized in situ using a custom piezoelectric inkjet system. The combination of chip-based staple strand production, double-sized origami, and high-yield one-pot assembly markedly increase the useful scale of DNA origami.
Authors:
Alexandria N Marchi; Ishtiaq Saaem; Jingdong Tian; Thomas H Labean
Related Documents :
7994107 - The use of microsatellites for genetic analysis of natural populations.
8094307 - Mhc class iii polymorphisms in selection of donors for bmt.
9608687 - Effect of reference database on frequency estimates of polymerase chain reaction (pcr)-...
8934697 - Restriction fragment length polymorphism (rflp) analysis on dna from human compact bone.
3118197 - Unique dna repair properties of a xeroderma pigmentosum revertant.
8330357 - The role of hepatocyte heterogeneity in the initiation of hepatocarcinogenesis.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-2
Journal Detail:
Title:  ACS nano     Volume:  -     ISSN:  1936-086X     ISO Abbreviation:  ACS Nano     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101313589     Medline TA:  ACS Nano     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A linear-time algorithm for reconstructing zero-recombinant haplotype configuration on a pedigree.
Next Document:  Persistent wandering atrial pacemaker after epinephrine overdosing - a case report.