| Oncostatin M synergises with house dust mite proteases to induce the production of PGE(2) from cultured lung epithelial cells. | |
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MedLine Citation:
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PMID: 11015296 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The release of PGE(2) and nitric oxide (NO) from the respiratory epithelium may act to dampen inflammation. In other tissues, oncostatin M (OSM), a potent inducer of epithelial antiproteases, has also been shown to interact with IL-1beta to stimulate PGE(2) release. However, whether OSM interacts with pro-inflammatory cytokines and proteases in the production of anti-inflammatory eicosanoids and NO from airway epithelium is unknown. The effect of OSM and the related cytokine leukaemia inhibitory factor (LIF) on PGE(2) and NO production by the respiratory epithelial cell line, A549 in response to pro-inflammatory cytokines as well as protease-rich house dust mite (HDM) fractions and a protease-deficient rye grass pollen extract was examined by immunohistochemistry, cell culture, ELISA and enzyme-immunoassay. Cells treated with a mixture of IL-1beta, IFNgamma and LPS for 48 h produced a 9 fold increase in PGE(2) and a 3 fold increase in NO levels (both P<0.05). Both OSM and LIF were without effect. However, OSM added together with the cytokine mixture synergistically enhanced PGE(2) production (22 fold, P<0.05). OSM also synergistically enhanced PGE(2) production in response to a cysteine protease-enriched, but not serine protease-enriched HDM fraction (P<0.05). Rye grass extract, neither alone nor in combination with OSM, induced PGE(2) or NO production, although it did induce the release of GM-CSF. These observations suggest that OSM is an important co-factor in the release of PGE(2) and NO from respiratory epithelial cells and may play a role in defense against exogenous proteases such as those derived from HDM. |
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Authors:
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D A Knight; N Asokananthan; D N Watkins; N L Misso; P J Thompson; G A Stewart |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: British journal of pharmacology Volume: 131 ISSN: 0007-1188 ISO Abbreviation: Br. J. Pharmacol. Publication Date: 2000 Oct |
Date Detail:
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Created Date: 2000-11-06 Completed Date: 2001-02-15 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7502536 Medline TA: Br J Pharmacol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 465-72 Citation Subset: IM |
Affiliation:
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Asthma & Allergy Research Institute, University of Western Australia, Nedlands, Western Australia. dknight@cyllene.uwa.edu.au |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Cytokines / metabolism Dinoprostone / metabolism* Drug Synergism Endopeptidases / pharmacology* Epithelial Cells / drug effects, metabolism Growth Inhibitors / metabolism Humans Immunohistochemistry Interleukin-6* Leukemia Inhibitory Factor Leukemia Inhibitory Factor Receptor alpha Subunit Lolium / chemistry Lung / cytology, drug effects*, metabolism Lymphokines / metabolism Mites / enzymology Nitric Oxide / metabolism Oncostatin M Peptides / pharmacology* Plant Extracts / pharmacology Pollen / chemistry Receptors, Cytokine / analysis Receptors, OSM-LIF Receptors, Oncostatin M |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Growth Inhibitors; 0/Interleukin-6; 0/LIF protein, human; 0/LIFR protein, human; 0/Leukemia Inhibitory Factor; 0/Leukemia Inhibitory Factor Receptor alpha Subunit; 0/Lymphokines; 0/OSM protein, human; 0/Peptides; 0/Plant Extracts; 0/Receptors, Cytokine; 0/Receptors, OSM-LIF; 0/Receptors, Oncostatin M; 10102-43-9/Nitric Oxide; 106956-32-5/Oncostatin M; 363-24-6/Dinoprostone; EC 3.4.-/Endopeptidases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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