Document Detail


Oncogenic transformation of C3H 10T1/2 cells exposed to alpha particles: sensitivity through the cell cycle.
MedLine Citation:
PMID:  7761577     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oncogenic transformation of synchronized C3H 10T1/2 cells was determined after exposure to 4.3 MeV alpha particles (LET = 101 keV/microns). Two synchronization techniques were tested using basic and modified protocols: one based on the release of cells from contact inhibition and the second on the mitotic shake-off method. Progression of cells through the cycle was followed as a function of time by flow cytometric analysis, DNA labeling for passage through S phase, the growth curve for the cell number and mitotic index measurements. The conclusion is that, although the release of cells from confluence provides higher yields of synchronized cells, mitotic shake-off proved to be the best way of collecting a synchronized population of minimally perturbed cells. Cells synchronized by mitotic shake-off were irradiated with 0.30 Gy in the interval between 2 and 10 h corresponding to G1 and early S phases. For comparison asynchronous populations were irradiated in parallel. Oncogenic transformation frequency, corrected for background, in mid-G1 phase was (18 +/- 4) x 10(-5) (average values of frequencies at 4 and 6 h) compared with the value of (8 +/- 4) x 10(-5) for the asynchronous population. While these data are suggestive of a trend toward a slightly increased sensitivity in mid-G1 phase, it is not statistically significant. The surviving fraction is constant in G1 phase.
Authors:
D Bettega; P Calzolari; A Costa; G N Chiorda; L Tallone
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Radiation research     Volume:  142     ISSN:  0033-7587     ISO Abbreviation:  Radiat. Res.     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1995-06-29     Completed Date:  1995-06-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  276-80     Citation Subset:  IM; S    
Affiliation:
Dipartimento di Fisica dell'Università degli Studi, Milan, Italy.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Alpha Particles*
Animals
Cell Cycle / radiation effects*
Cell Survival / radiation effects
Cell Transformation, Neoplastic*
Flow Cytometry
Kinetics
Mice
Mice, Inbred C3H
Mitosis / radiation effects
Mitotic Index / radiation effects
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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