| Oncogenic H-Ras V12 promotes anchorage-independent cytokinesis in human fibroblasts. | |
| | |
MedLine Citation:
|
PMID: 18077377 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cell anchorage is required for cell proliferation of untransformed cells, whereas anchorage-independent growth can be induced by oncogenes and is a hallmark of transformation. Whereas anchorage-dependent control of the progression of the G(1) phase of the cell cycle has been extensively studied, it is less clear whether and how anchorage may control other cell cycle phases and whether oncogenes may affect such controls. Here, we found that lack of cell anchorage did not influence progression through the cell cycle S phase, G(2) phase, or most of mitosis of primary human fibroblasts. However, unanchored fibroblasts could not complete cytokinesis. The cleavage furrow and central spindle were still formed in the absence of anchorage, but cells were unable to complete ingression, causing binucleation. Importantly, V12 H-Ras-transformed fibroblasts and two cancer cell lines progressed through the entire cell cycle without anchorage, including through cytokinesis. This indicates that oncogenic signaling may contribute to anchorage-independent growth and tumorigenesis by promoting the final cleavage furrow ingression during cytokinesis. |
| | |
Authors:
|
Minna Thullberg; Annica Gad; Sylvie Le Guyader; Staffan Strömblad |
Related Documents
:
|
9728657 - Paclitaxel-induced modification of the effects of radiation and alterations in the cell... 2756117 - The radiation response of cells recovering after chronic hypoxia. 1352437 - The biochemistry of the neuron: methods and calculations for the analysis of neurotrans... 22695187 - Tlr9 drives the development of transitional b cells towards the marginal zone pathway a... 17719097 - A carboxymethyl-cellulose plaque assay for feline calicivirus. 9022147 - Expression of alpha2-macroglobulin receptor/low density lipoprotein receptor-related pr... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-12-11 |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 104 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2007 Dec |
Date Detail:
|
Created Date: 2007-12-20 Completed Date: 2008-01-28 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
|
Languages: eng Pagination: 20338-43 Citation Subset: IM |
Affiliation:
|
Department of Biosciences and Nutrition, Karolinska Institutet, SE-141 57 Huddinge, Sweden. minna.thullberg@ki.se |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Adhesion*
/
genetics Cell Line, Tumor Cell Proliferation Cell Transformation, Neoplastic / genetics* Cytokinesis* / genetics Fibroblasts / cytology Genes, ras / physiology* Humans Oncogenes / physiology* |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Retrograde endocannabinoid signaling at striatal synapses requires a regulated postsynaptic release ...
Next Document: Estimation of multiple transmission rates for epidemics in heterogeneous populations.