Document Detail

Oncogene expression in differentiating F9 mouse embryonal carcinoma cells.
MedLine Citation:
PMID:  3691668     Owner:  NLM     Status:  MEDLINE    
Differentiation of F9 mouse embryonal carcinoma cells in culture is accompanied by a decrease in growth rate and loss of tumorigenicity. Cells differentiating in monolayer culture (to parietal endoderm-type cells) or in aggregates (to visceral endoderm-type cells) show qualitatively similar changes in transcript levels from several c-oncogenes. In contrast with other studies with F9 cells, we find an early decrease in c-myb RNA but not in c-myc RNA. This and a later increase in c-src RNA may be associated with decreasing cell growth rate. Before differentiation, induction and maintenance of elevated c-abl RNA levels depend on the presence of retinoic acid in the medium. After differentiation c-abl RNA levels decline only partially when retinoic acid is removed. Increased RNA from c-fos is seen late in differentiation in monolayer cultures only, a change also seen with appearance of similar endoderm cell types in the developing mouse embryo.
T J Lockett; M J Sleigh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  173     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1987 Dec 
Date Detail:
Created Date:  1988-02-02     Completed Date:  1988-02-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  370-8     Citation Subset:  IM    
CSIRO Division of Molecular Biology, New South Wales, Australia.
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MeSH Terms
Cell Transformation, Neoplastic / analysis,  metabolism*
Embryonal Carcinoma Stem Cells
Gene Expression Regulation*
Neoplastic Stem Cells / analysis,  metabolism*
RNA, Neoplasm / metabolism
Time Factors
Tumor Cells, Cultured / analysis,  metabolism*
Reg. No./Substance:
0/RNA, Neoplasm

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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