Document Detail


Oncogene alterations in in vitro transformed rat tracheal epithelial cells.
MedLine Citation:
PMID:  1691445     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
10 derivations of rat tracheal epithelial (RTE) cells, including normal cells, normal primary cultures, 7 tumorigenic cell lines and 1 nontumorigenic cell line transformed in vitro by treatment with 7,12-dimethylbenz[a]anthracene (DMBA), benzo[a]pyrene (BP) and/or 12-O-tetradecanoylphorbol-13-acetate (TPA) were examined for oncogene alterations. No abnormalities of Ha-ras or Ki-ras were seen that were suggestive of amplification, rearrangement or the presence of RFLPs. Analysis of specific-point mutations in Ha-ras using Pst I digestion (codon 12, GGA to GCA) or Ha-ras and Ki-ras using Xba I (codon 61, CAA to CTA) were negative. In one cell line derived by DMBA treatment, changes in the c-myc restriction digest pattern were seen after incubation with Bam HI and Hind III. Northern analysis revealed consistent differences between normal and transformed cells when probed with Ha-ras; c-myc expression was of low intensity, and the expression of Ki-ras could not be detected. Transfection of RTE cell DNAs into NIH/3T3 cells did not result in the appearance of morphologic transformants. The studies suggest that Ha-ras or Ki-ras codon 61 A to T transversions (CAA to CTA) are not associated with the immortal/tumorigenic phenotype in RTE cells transformed by DMBA or TPA, and are in contrast to results reported in some other biological systems.
Authors:
M J Mass; N S Schorschinsky; J A Lasley; D K Beeman; S J Austin
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Mutation research     Volume:  243     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1990 Apr 
Date Detail:
Created Date:  1990-05-15     Completed Date:  1990-05-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  291-8     Citation Subset:  IM    
Affiliation:
Carcinogenesis and Metabolism Branch, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711.
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MeSH Terms
Descriptor/Qualifier:
9,10-Dimethyl-1,2-benzanthracene
Animals
Blotting, Northern
Cell Line
Cell Transformation, Neoplastic / genetics*
DNA / drug effects,  genetics*
Epithelium
Gene Amplification
Gene Rearrangement
Genes, ras / genetics*
Male
Nucleic Acid Hybridization
Oncogene Protein p21(ras) / genetics
Oncogenes / drug effects,  genetics*
Polymorphism, Restriction Fragment Length
Proto-Oncogene Proteins / genetics
Proto-Oncogene Proteins c-myc
RNA / drug effects,  genetics
Rats
Tetradecanoylphorbol Acetate
Trachea / cytology
Transfection
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-myc; 16561-29-8/Tetradecanoylphorbol Acetate; 57-97-6/9,10-Dimethyl-1,2-benzanthracene; 63231-63-0/RNA; 9007-49-2/DNA; EC 3.6.5.2/Oncogene Protein p21(ras)

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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