Document Detail


OmpR positively regulates urease expression to enhance acid survival of Yersinia pseudotuberculosis.
MedLine Citation:
PMID:  19443542     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Yersinia pseudotuberculosis is an enteric bacterium which must overcome the acidic stress in host organs for successful colonization, but how this bacterium survives in acidic conditions remains largely unknown. In the present study, the importance of OmpR in acid survival of Y. pseudotuberculosis YpIII was confirmed by the fact that mutation of ompR (strain DeltaompR) greatly reduced cell survival at pH 4.5 or lower. To characterize the regulatory role of OmpR in this acid survival process, proteomic analysis was carried out to compare YpIII at pH 7.0 and pH 4.5 with DeltaompR at pH 7.0, and urease components were revealed to be the main targets for OmpR regulation. Addition of urea to the culture medium also enhanced acid survival of YpIII but not DeltaompR and urease activity was significantly induced by acid in YpIII but not in DeltaompR. Each of the seven components of the YpIII urease gene cluster was fused to a lacZ reporter and their expression was dramatically decreased in a DeltaompR background; this supports the notion that OmpR positively regulates urease expression. Furthermore, gel shift analysis revealed that OmpR binds to the deduced promoter regions of three polycistronic transcriptional units (ureABC, ureEF and ureGD) in the urease cluster, suggesting that the regulation of OmpR to urease components is direct. Taken together, these data strongly suggest that OmpR activates urease expression to enhance acid survival in Y. pseudotuberculosis.
Authors:
Yangbo Hu; Pei Lu; Yao Wang; Lisha Ding; Steve Atkinson; Shiyun Chen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-14
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  155     ISSN:  1350-0872     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-29     Completed Date:  2009-10-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  England    
Other Details:
Languages:  eng     Pagination:  2522-31     Citation Subset:  IM    
Affiliation:
Wuhan Institute of Virology, The Chinese Academy of Sciences, Wuhan 430071, PR China.
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MeSH Terms
Descriptor/Qualifier:
Acids / metabolism*
Bacterial Proteins / metabolism*
DNA, Bacterial / genetics
Electrophoresis, Gel, Two-Dimensional
Electrophoretic Mobility Shift Assay
Gene Expression Regulation, Bacterial
Hydrogen-Ion Concentration
Multigene Family
Promoter Regions, Genetic
Stress, Physiological
Trans-Activators / metabolism*
Transcription, Genetic
Urease / biosynthesis*,  genetics
Yersinia pseudotuberculosis / physiology*
Chemical
Reg. No./Substance:
0/Acids; 0/Bacterial Proteins; 0/DNA, Bacterial; 0/Trans-Activators; 0/osmolarity response regulator proteins; EC 3.5.1.5/Urease

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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