Document Detail


Omeprazole and regulation of cytokine profile in Helicobacter pylori-infected patients with duodenal ulcer disease.
MedLine Citation:
PMID:  11100337     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/AIMS: To estimate the cytokine profile in Helicobacter pylori-positive patients with duodenal ulcers treated with omeprazole. METHODOLOGY: The subjects were 22 patients with endoscopically defined active duodenal ulcers and H. pylori infection treated with omeprazole for 3 months after initial 1-week triple therapy. Peripheral blood CD3+ CD4+ and CD8+ T lymphocytes, percentage of HLA-DR+ peripheral blood lymphocytes, and cytokine levels (GM-CSF, IL-6 and IL-2) were measured in the supernatants from PHA-cultured peripheral blood lymphocytes at baseline conditions and after 3-months' omeprazole treatment. RESULTS: The H. pylori eradication triple regimen cured H. pylori infection in 86.4% of our patients. Peripheral blood CD3+ CD4+ and CD8+ T lymphocytes were not affected by omeprazole treatment (P > 0.05), whereas the percentage of HLA-DR+ peripheral blood lymphocytes increased significantly post-treatment (P < 0.05). All cytokine levels measured in the supernatants showed a significant decrease after treatment (P < 0.001). CONCLUSIONS: The significant decrease in the two pro-inflammatory cytokines (GM-CSF, IL-6) and the major inflammatory cytokine IL-2, implies that the potential cytokine synthesis of the Th1 pattern from peripheral blood lymphocytes is highly suppressed after treatment. This effect on cytokine regulation seems to be a result of the positive H. pylori infection outcome at the immunological level, which leads to "normalization" of cytokine production.
Authors:
J Kountouras; P Boura; N J Lygidakis
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Hepato-gastroenterology     Volume:  47     ISSN:  0172-6390     ISO Abbreviation:  Hepatogastroenterology     Publication Date:    2000 Sep-Oct
Date Detail:
Created Date:  2001-03-29     Completed Date:  2001-05-31     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8007849     Medline TA:  Hepatogastroenterology     Country:  GREECE    
Other Details:
Languages:  eng     Pagination:  1301-4     Citation Subset:  IM    
Affiliation:
Department of Medicine, Aristotle University of Thessaloniki, Ippokration Hospital, Greece. jannis@med.auth.gr
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MeSH Terms
Descriptor/Qualifier:
Anti-Ulcer Agents / pharmacology,  therapeutic use*
Duodenal Ulcer / complications,  drug therapy*,  immunology*
Female
Granulocyte-Macrophage Colony-Stimulating Factor / blood*
Helicobacter Infections / complications,  drug therapy*,  immunology*
Helicobacter pylori*
Humans
Interleukin-2 / blood*
Interleukin-6 / blood*
Male
Middle Aged
Omeprazole / pharmacology,  therapeutic use*
T-Lymphocyte Subsets / immunology
Chemical
Reg. No./Substance:
0/Anti-Ulcer Agents; 0/Interleukin-2; 0/Interleukin-6; 73590-58-6/Omeprazole; 83869-56-1/Granulocyte-Macrophage Colony-Stimulating Factor

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