Document Detail


Omegacoeur, a Mediterranean nutritional complement, stimulates Na,K-ATPase activity in human endothelial cells.
MedLine Citation:
PMID:  11355006     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fatty acids are known as modulators of the vasoactive properties of the vessel wall and can influence the physical and functional properties of cell membrane. The membrane-bound enzyme Na,K-ATPase plays a central role in endothelial function such as vasoconstriction. In a previous study, we have shown that omega3 fatty acids inhibited Na,K-ATPase activity in human endothelial cells. As Mediterranean diet is known to protect from cardiovascular diseases, we have investigated the effects of Omegacoeur, a Mediterranean nutritional complement consisting of omega3, omega6, omega9 fatty acids, garlic and basil, on Na,K-ATPase activity in human endothelial cells (HUVECs). Cells were incubated for 18 hr with pure lecithin liposomes or Omegacoeur-enriched emulsions (4 mg lecithin/ml). Na,K-ATPase and 5'-nucleotidase activities were determined using coupled assay methods on microsomal fractions obtained from HUVECs. Cell fatty acid composition was evaluated by gas chromatography after extraction of lipids and fatty acids methylation. The results showed that Omegacoeur (0.1 mM) increased Na,K-ATPase activity by 40% without changes in 5'-nucleotidase activity. Cells incubated with Omegacoeur preferentially incorporated linoleic acid. Therefore, linoleic acid or others constituents of Omegacoeur could be responsible of the stimulation of the Na,K-ATPase activity that might be related to changes in endothelial membrane fluidity.
Authors:
M J Duran; F Sabatier; G Pieroni; G Gerber; J Sampol; J M Maixent
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cellular and molecular biology (Noisy-le-Grand, France)     Volume:  47     ISSN:  0145-5680     ISO Abbreviation:  Cell. Mol. Biol. (Noisy-le-grand)     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-05-16     Completed Date:  2001-12-04     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9216789     Medline TA:  Cell Mol Biol (Noisy-le-grand)     Country:  France    
Other Details:
Languages:  eng     Pagination:  313-8     Citation Subset:  IM    
Affiliation:
Laboratoire de Biochimie Fondamentale, Moléculaire et Clinique, Faculté de Pharmacie, Marseille, France.
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MeSH Terms
Descriptor/Qualifier:
5'-Nucleotidase / metabolism
Cell Fractionation
Cells, Cultured
Culture Media, Serum-Free
Dietary Supplements*
Endothelium, Vascular / cytology,  drug effects,  enzymology*
Fatty Acids / metabolism*
Humans
Liposomes / chemistry,  metabolism
Mediterranean Region
Microsomes / chemistry,  metabolism
Phosphatidylcholines / chemistry,  pharmacology
Sodium-Potassium-Exchanging ATPase / metabolism*
Chemical
Reg. No./Substance:
0/Culture Media, Serum-Free; 0/Fatty Acids; 0/Liposomes; 0/Phosphatidylcholines; EC 3.1.3.5/5'-Nucleotidase; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase

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