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[Omega-6 polyunsaturated fatty acid promotes colon carcinogenesis].
MedLine Citation:
PMID:  20972911     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
OBJECTIVE: To investigate whether ω-6 polyunsaturated fatty acid promotes colon carcinogenesis through downregulation of P53-dependent growth inhibition.
METHODS: Colon carcinogenesis was induced by injection of azoxymethane (AOM) intraperitoneally. Experimental animals were randomly divided into four groups, receiving regular diet and intraperitoneal injection of normal saline(control group), high ω-6 polyunsaturated fatty acid diet with intraperitoneal injection of normal saline(Corn oil group), regular diet with intraperitoneal injection of AOM(AOM group), or high ω-6 polyunsaturated fatty acid diet with intraperitoneal injection of AOM (Corn oil+AOM group). Aberrant crypt focis (ACFs) were observed after methylene blue staining and enumerated. Colonic mucosa PCNA and P53 expressions were assessed by RT-PCR and Western blotting, and location of P53 in the colon crypt focis was determined by immunohistochemical staining.
RESULTS: Amounts of ACFs was 1.2±0.3 in the control group, 1.3±0.4 in the Corn oil group, 41.0±4.8 in the AOM group, and 73.3±9.9 in the Corn oil+AOM group, the differences were statistically significant(P<0.05). The expression of P53 in normal crypt focis was higher than that in ACFs. High ω-6 polyunsaturated fatty acid dietary significantly promoted AOM-induced colon PCNA expression, and enhanced AOM-mediated P53 inhibition in colon mucosa.
CONCLUSION: High ω-6 polyunsaturated fatty acid diet can enhance AOM-induced inhibition of P53 in colon mucosa, resulting in overexpression of PCNA, formation of ACF, and carcinogenesis in the colon.
Authors:
Xiao-liang Chen; Jian-zhong Li; Li-xian Zeng; Ya-shi Zhan; Ying-hui Yang; Qi Yang; Hui-ling Liu; Bin Wu
Publication Detail:
Type:  English Abstract; Journal Article    
Journal Detail:
Title:  Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery     Volume:  13     ISSN:  1671-0274     ISO Abbreviation:  Zhonghua Wei Chang Wai Ke Za Zhi     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101177990     Medline TA:  Zhonghua Wei Chang Wai Ke Za Zhi     Country:  China    
Other Details:
Languages:  chi     Pagination:  774-7     Citation Subset:  IM    
Affiliation:
Department of Gastroenterology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.
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