Document Detail

Omega-6 docosapentaenoic acid-derived resolvins and 17-hydroxydocosahexaenoic acid modulate macrophage function and alleviate experimental colitis.
MedLine Citation:
PMID:  22618200     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Enzymatically oxygenated lipid products derived from omega-3 and omega-6 fatty acids play an important role in inflammation dampening. This study examined the anti-inflammatory effects of n-6 docosapentaenoic acid-derived (17S)-hydroxy-docosapentaenoic acid (17-HDPAn-6) and (10,17S)-dihydroxy-docosapentaenoic acid (10,17-HDPAn-6) as well as n-3 docosahexaenoic acid-derived 17(R/S)-hydroxy-docosahexaenoic acid (17-HDHA).
MATERIALS AND METHODS: The effects of 17-HDPAn-6, 10,17-HDPAn-6 or 17-HDHA on activity and M1/M2 polarization of murine macrophage cell line RAW 264.7 were examined by phagocytosis assay and real-time PCR. To assess anti-inflammatory effects in vivo, dextran sodium sulfate (DSS) colitis was induced in mice treated with 17-HDPAn-6, 10,17-HDPAn-6, 17-HDHA or NaCl.
RESULTS: Our results show that 17-HDPAn-6, 10,17-HDPAn-6 and 17-HDHA increase phagocytosis in macrophages in vitro and promote polarization towards the anti-inflammatory M2 phenotype with decreased gene expression of TNF-α and inducible Nitric oxide synthase and increased expression of the chemokine IL-1 receptor antagonist and the Scavenger receptor Type A. Intraperitoneal treatment with 17-HDPAn-6, 10,17-HDPAn-6, or 17-HDHA alleviated DSS-colitis and significantly improved body weight loss, colon epithelial damage, and macrophage infiltration.
CONCLUSION: These results suggest that DPAn-6-derived 17-HDPAn-6 and 10,17-HDPAn-6 as well as the DHA-derived 17-HDHA have inflammation-dampening and resolution-promoting effects that could be used to treat inflammatory conditions such as inflammatory bowel disease.
Cheng-Ying Chiu; Beate Gomolka; Cordula Dierkes; Nora R Huang; Maik Schroeder; Martin Purschke; Dieter Manstein; Bindi Dangi; Karsten H Weylandt
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-23
Journal Detail:
Title:  Inflammation research : official journal of the European Histamine Research Society ... [et al.]     Volume:  61     ISSN:  1420-908X     ISO Abbreviation:  Inflamm. Res.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-14     Completed Date:  2013-01-03     Revised Date:  2013-02-13    
Medline Journal Info:
Nlm Unique ID:  9508160     Medline TA:  Inflamm Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  967-76     Citation Subset:  IM    
Department of Hepatology, Gastroenterology and Endocrinology, Charité University Medicine Berlin, Campus Virchow Klinikum, Berlin, Germany.
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MeSH Terms
Anti-Inflammatory Agents / pharmacology*,  therapeutic use
Cell Line
Colitis / drug therapy,  immunology*,  pathology
Disease Models, Animal
Docosahexaenoic Acids / pharmacology*,  therapeutic use
Fatty Acids, Unsaturated / pharmacology*,  therapeutic use
Macrophages / drug effects*,  physiology
Phagocytosis / drug effects
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Fatty Acids, Unsaturated; 25167-62-8/Docosahexaenoic Acids; 25448-00-4/docosapentaenoic acid; 90780-52-2/17-hydroxy-4,7,10,13,15,19-docosahexaenoic acid
Erratum In:
Inflamm Res. 2012 Nov;61(11):1293

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