| Omega-3E treatment regulates matrix metalloproteinases and prevents vascular reactivity alterations in diabetic rat aorta. | |
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MedLine Citation:
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PMID: 20029543 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It is known that increased generation of oxidants and (or) reduced endogenous antioxidant defense mechanisms are associated with the etiology of diabetic vascular complications. Although a close correlation exists between increased oxidative stress and the activation of matrix metalloproteinases (MMPs), little is known about the effect of hyperglycemia on the regulation and contribution of MMPs in the vascular system. Therefore, we aimed to examine whether omega-3E (50 mg/kg per day for 4 weeks), a long-chain (n-3) polyunsaturated fatty acid enriched with vitamin E, has a beneficial effect on vascular dysfunction via affecting MMPs in streptozotocin-diabetic rat aorta. Omega-3E treatment improved the diabetes-induced impairment of phenylephrine-induced contraction and isoproterenol-induced relaxation responses of aorta. It also exhibited marked protection against diabetes-induced degenerative changes in smooth muscle cell morphology. Biochemical data showed that this treatment significantly prevented important changes, such as inhibition of MMP-2 and MMP-9 activity, loss of tissue inhibitor of matrix metalloproteinase-4 (TIMP-4) protein, increase in tissue levels of thiol oxidation, endothelin-1, protein kinase C (PKC), and cAMP production, and decrease in tissue level of nitrite. These results indicated that omega-3E significantly improved impaired vascular responses and regulated the activity of MMPs via preventing oxidative injury. Overall, the data suggest that omega-3E ameliorates or prevents vascular reactivity alterations in diabetes. Such an observation provides preliminary evidence for omega-3E's potential as a therapeutic agent for the prevention of vascular disorders in diabetes. |
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Authors:
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Esma N Zeydanli; Belma Turan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Canadian journal of physiology and pharmacology Volume: 87 ISSN: 1205-7541 ISO Abbreviation: Can. J. Physiol. Pharmacol. Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-12-23 Completed Date: 2010-03-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372712 Medline TA: Can J Physiol Pharmacol Country: Canada |
Other Details:
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Languages: eng Pagination: 1063-73 Citation Subset: IM |
Affiliation:
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Department of Biophysics, Faculty of Medicine, Ankara University, Ankara 06100, Turkey. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta, Thoracic / chemistry, drug effects, physiopathology* Blotting, Western Cyclic AMP / analysis Diabetes Mellitus, Experimental / drug therapy, physiopathology* Diabetic Angiopathies / drug therapy, physiopathology*, prevention & control Fatty Acids, Omega-3 / pharmacology* Male Matrix Metalloproteinase 2 / drug effects*, metabolism, physiology Matrix Metalloproteinase 9 / drug effects*, metabolism, physiology Muscle, Smooth, Vascular / drug effects, physiopathology Nitrites / analysis Rats Rats, Wistar Vitamin E / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Omega-3; 0/Nitrites; 1406-18-4/Vitamin E; 60-92-4/Cyclic AMP; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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