Document Detail


Olomoucine, an inhibitor of the cdc2/cdk2 kinases activity, blocks plant cells at the G1 to S and G2 to M cell cycle transitions.
MedLine Citation:
PMID:  7523194     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cdc2/cdk2 protein kinases play key roles in the cell cycle at two control points: the G1/S transition and the entry into mitosis. Olomoucine, a specific inhibitor of these kinases, was tested in two plant cell systems: Petunia mesophyll protoplasts induced to divide and Arabidopsis thaliana cell suspension cultures. The cell cycle status was analysed from DNA histograms or through continuous labelling of cells with 5-bromodeoxyuridine (BrdUrd) followed by double staining with bis-benzimide (Hoechst 33258) and propidium iodide (PI). Such analyses resolve cells from several generations according to the extent of their DNA replication. Olomoucine was shown to reversibly arrest differentiated Petunia cells induced to divide at G1 phase and cycling Arabidopsis cells in late G1 and G2. A comparison of the effects of aphidicolin, oryzalin and olomoucine suggests that in the Arabidopsis cell suspension culture, a cdc2/cdk2-like kinase is activated at a restriction point in late G1.
Authors:
N Glab; B Labidi; L X Qin; C Trehin; C Bergounioux; L Meijer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  353     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1994-11-23     Completed Date:  1994-11-23     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  207-11     Citation Subset:  IM    
Affiliation:
Laboratoire de Physiologie Végétale Moléculaire CNRS, URA 1128, Faculté des Sciences, Orsay, France.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / cytology
Bisbenzimidazole
Bromodeoxyuridine / metabolism
CDC2 Protein Kinase / antagonists & inhibitors*
CDC2-CDC28 Kinases*
Cell Cycle / drug effects*
Cells, Cultured
Cyclin-Dependent Kinase 2
Cyclin-Dependent Kinases*
G1 Phase / drug effects
G2 Phase / drug effects
Kinetin
Mitosis / drug effects
Plants / cytology*,  drug effects
Propidium
Protein-Serine-Threonine Kinases / antagonists & inhibitors*
Purines / pharmacology*
S Phase / drug effects
Staining and Labeling
Chemical
Reg. No./Substance:
0/Purines; 0/olomoucine; 23491-44-3/Bisbenzimidazole; 36015-30-2/Propidium; 525-79-1/Kinetin; 59-14-3/Bromodeoxyuridine; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.22/CDC2 Protein Kinase; EC 2.7.11.22/CDC2-CDC28 Kinases; EC 2.7.11.22/Cyclin-Dependent Kinase 2; EC 2.7.11.22/Cyclin-Dependent Kinases

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