| Olomoucine, an inhibitor of the cdc2/cdk2 kinases activity, blocks plant cells at the G1 to S and G2 to M cell cycle transitions. | |
| | |
MedLine Citation:
|
PMID: 7523194 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The cdc2/cdk2 protein kinases play key roles in the cell cycle at two control points: the G1/S transition and the entry into mitosis. Olomoucine, a specific inhibitor of these kinases, was tested in two plant cell systems: Petunia mesophyll protoplasts induced to divide and Arabidopsis thaliana cell suspension cultures. The cell cycle status was analysed from DNA histograms or through continuous labelling of cells with 5-bromodeoxyuridine (BrdUrd) followed by double staining with bis-benzimide (Hoechst 33258) and propidium iodide (PI). Such analyses resolve cells from several generations according to the extent of their DNA replication. Olomoucine was shown to reversibly arrest differentiated Petunia cells induced to divide at G1 phase and cycling Arabidopsis cells in late G1 and G2. A comparison of the effects of aphidicolin, oryzalin and olomoucine suggests that in the Arabidopsis cell suspension culture, a cdc2/cdk2-like kinase is activated at a restriction point in late G1. |
| | |
Authors:
|
N Glab; B Labidi; L X Qin; C Trehin; C Bergounioux; L Meijer |
Related Documents
:
|
1505524 - Cell cycle regulation of glucocorticoid receptor function. 3137384 - Diverse effects of adp-ribosyl transferase inhibitors on g2 progression and lethality i... 17976834 - Inhibition of toxoplasma gondii growth by pyrrolidine dithiocarbamate is cell cycle spe... 4066754 - Control of myogenic differentiation by fibroblast growth factor is mediated by position... 3047414 - Alterations of the posttranslational processing of a lysosomal enzyme in c6 glioma cells. 20335794 - Induction of apoptosis and inhibition of prostate and breast cancer growth by bgp-15, a... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: FEBS letters Volume: 353 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1994 Oct |
Date Detail:
|
Created Date: 1994-11-23 Completed Date: 1994-11-23 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 207-11 Citation Subset: IM |
Affiliation:
|
Laboratoire de Physiologie Végétale Moléculaire CNRS, URA 1128, Faculté des Sciences, Orsay, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Arabidopsis
/
cytology Bisbenzimidazole Bromodeoxyuridine / metabolism CDC2 Protein Kinase / antagonists & inhibitors* CDC2-CDC28 Kinases* Cell Cycle / drug effects* Cells, Cultured Cyclin-Dependent Kinase 2 Cyclin-Dependent Kinases* G1 Phase / drug effects G2 Phase / drug effects Kinetin Mitosis / drug effects Plants / cytology*, drug effects Propidium Protein-Serine-Threonine Kinases / antagonists & inhibitors* Purines / pharmacology* S Phase / drug effects Staining and Labeling |
| Chemical | |
Reg. No./Substance:
|
0/Purines; 0/olomoucine; 23491-44-3/Bisbenzimidazole; 36015-30-2/Propidium; 525-79-1/Kinetin; 59-14-3/Bromodeoxyuridine; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.22/CDC2 Protein Kinase; EC 2.7.11.22/CDC2-CDC28 Kinases; EC 2.7.11.22/Cyclin-Dependent Kinase 2; EC 2.7.11.22/Cyclin-Dependent Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Isolation and characterization of mutants of human mitogen-activated protein kinase (ERK2).
Next Document: Collagen stimulates tyrosine phosphorylation of phospholipase C-gamma 2 but not phospholipase C-gamm...