Document Detail

Oligosaccharides from human milk influence growth-related characteristics of intestinally transformed and non-transformed intestinal cells.
MedLine Citation:
PMID:  17925055     Owner:  NLM     Status:  MEDLINE    
Human milk oligosaccharides (HMO) are considered to influence the composition of the gut microflora in breastfed infants. We investigated direct effects of milk HMO fractions or individual oligosaccharides on proliferation, differentiation and apoptosis in transformed human intestinal cells (HT-29 and Caco-2) and non-transformed small intestinal epithelial crypt cells of fetal origin (human intestinal epithelial cells; HIEC). We observed growth inhibition induced by neutral and acidic HMO fractions in HT-29, Caco-2 and HIEC cells in a dose dependent manner. However, the effects varied between cell lines, i.e. HT-29 and Caco-2 cells were more sensitive than HIEC cells. In HT-29, all 16 individual neutral and acidic oligosaccharides except from the two fucosyllactoses had an inhibitory effect on cell growth. Regarding the induction of differentiation in HT-29 and HIEC cells a threshold concentration was observed at 7.5 mg/ml for neutral and acidic HMO fractions. Among individual oligosaccharides, only sialyllactoses induced differentiation in HT-29 and HIEC cells; no effect neither of fractions nor of individual oligosaccharides was found in Caco-2 cells. A strong induction of apoptosis was only detected in HT-29 and HIEC cells for neutral oligosaccharide but not for acidic fractions. HMO were shown to induce growth inhibition in intestinal cells through two different mechanisms, by suppressing cell cycle progression through induction of differentiation and/or by influencing apoptosis. As the development and maturation of digestive and absorptive processes depend on differentiation our experiments show that oligosaccharides are effective at influencing various stages in gastrointestinal development in vitro.
Sabine Kuntz; Silvia Rudloff; Clemens Kunz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-10
Journal Detail:
Title:  The British journal of nutrition     Volume:  99     ISSN:  0007-1145     ISO Abbreviation:  Br. J. Nutr.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-07     Completed Date:  2008-04-04     Revised Date:  2009-05-07    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  462-71     Citation Subset:  IM    
Institute of Nutritional Science, Justus-Liebig-University Giessen, Wilhelmstrasse 20, D-35392 Giessen, Germany.
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MeSH Terms
Alkaline Phosphatase / metabolism
Apoptosis / drug effects
Caco-2 Cells
Cell Differentiation / drug effects
Cell Proliferation / drug effects
Cell Transformation, Neoplastic
Cells, Cultured
Dietary Carbohydrates / pharmacology*
Dose-Response Relationship, Drug
HT29 Cells
Hydrogen-Ion Concentration
Intestinal Mucosa / cytology,  drug effects,  enzymology
Milk, Human / chemistry*
Oligosaccharides / pharmacology*
Reg. No./Substance:
0/Dietary Carbohydrates; 0/Oligosaccharides; EC Phosphatase
Comment In:
Br J Nutr. 2009 May;101(9):1267-9   [PMID:  19079836 ]

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